Analysis of hydrogen peroxide in an aqueous extract of cigarette smoke and effect of pH on the yield

An analysis of hydrogen peroxide in an aqueous extract of cigarette smoke, which contains many redox-active compounds, requires a method with high selectivity. An aqueous extract of the particulate phase of cigarette smoke was analyzed by HPLC with an electrochemical detector (ECD). Samples were pre...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-10, Vol.73 (10), p.2222-2225
Main Authors: Takanami, Y.(Japan Tobacco Inc., Yokohama), Moriyama, T, Kosaka, Y, Nakayama, T
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical Fractionation - methods
cigarette smoke
electrochemical detection
ESPECIES DE OXIGENO REACTIVO
EXTRACTOS
EXTRACTS
EXTRAIT
FUMEE
Fundamental and applied biological sciences. Psychology
HPLC
HUMO
HYDROGEN PEROXIDE
Hydrogen Peroxide - analysis
Hydrogen Peroxide - isolation & purification
Hydrogen-Ion Concentration
Injections
PEROXIDO DE HIDROGENO
PEROXYDE D'HYDROGENE
REACTIVE OXYGEN SPECIES
Smoke - analysis
SMOKES
Smoking
TABAC
TABACO
TOBACCO
Water - chemistry
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Summary:An analysis of hydrogen peroxide in an aqueous extract of cigarette smoke, which contains many redox-active compounds, requires a method with high selectivity. An aqueous extract of the particulate phase of cigarette smoke was analyzed by HPLC with an electrochemical detector (ECD). Samples were prepared by collecting the particulate phase of the cigarette smoke on a glass fiber filter and extracting it with a phosphate buffer. The obtained solution was purified by using a Waters Oasis MCX cation-exchange cartridge, and then analyzed by an HPLC-ECD system with a Shodex KS-801 mixed-mode resin column. Pre-injecting hydrogen peroxide at a high concentration into the HPLC instrument stabilized the analytical results. The recovery of hydrogen peroxide by using an extract of the particulate phase of the cigarette smoke was more than 80%. An increase in the amount of hydrogen peroxide was observed during extraction with the phosphate buffer at higher pH values. In contrast, extraction with phosphoric acid did not increase the amount of hydrogen peroxide during extraction.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.90324