Retrospective Diagnostics of Congenital Cytomegalovirus Infection Performed by Polymerase Chain Reaction in Blood Stored on Filter Paper

Capillary blood samples from 63 infants collected 3-7 days after birth, and thereafter stored on filter papers for 12-18 y, were tested for the presence of CMV DNA by the polymerase chain reaction (PCR) method. Of 16 infants with proven congenital CMV infection (positive virus isolation test in urin...

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Bibliographic Details
Published in:Scandinavian journal of infectious diseases 1997, Vol.29 (5), p.465-468
Main Authors: Hugo Johansson, P. J., Jönsson, Marianne, Ahlfors, Karin, Ivarsson, Sten A., Svanberg, Lars, Guthenberg, Claes
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blood Specimen Collection
Cytomegalovirus - isolation & purification
Cytomegalovirus Infections - blood
Cytomegalovirus Infections - congenital
DNA, Viral - analysis
Human viral diseases
Humans
Infant, Newborn
Infectious diseases
Medical sciences
Miscellaneous
Polymerase Chain Reaction
Time Factors
Viral diseases
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Summary:Capillary blood samples from 63 infants collected 3-7 days after birth, and thereafter stored on filter papers for 12-18 y, were tested for the presence of CMV DNA by the polymerase chain reaction (PCR) method. Of 16 infants with proven congenital CMV infection (positive virus isolation test in urine sampled within 1 week of age), 13 (81%) had a positive CMV PCR test and 3 (19%) a negative PCR test. All blood samples from 16 control infants without congenital CMV infection (negative virus isolation test in urine sampled within 1 week of age) were CMV PCR-negative. When 31 samples on filter papers stored above or below the samples of the infected infants were tested, 6 (19%) had a weak reactivity. This suggests that CMV DNA can be transferred from one filter paper to another during storage. We conclude that PCR performed on dried blood stored on filter paper is a useful method in the retrospective diagnostics of congenital CMV infection. Consideration must be given, however, to the possibility of transfer of CMV DNA from blood samples stored nearby.
ISSN:0036-5548
1651-1980
DOI:10.3109/00365549709011855