Genomic Organization of the Human Metabotropic Glutamate Receptor Subtype 3

In this study, the genomic organization of the human metabotropic glutamate receptor subtype 3 (mGluR3) gene has been determined. We have identified two transcription initiation sites and the polyadenylation signal by using 5′-rapid amplification of cDNA ends (RACE) and 3′-RACE, respectively. The ex...

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Bibliographic Details
Published in:Journal of neurogenetics 2000-01, Vol.14 (4), p.207-225
Main Authors: Corti, Corrado, Sala, Cinzia F., Yang, Fentang, Corsi, Mauro, Xuereb, John H., Ferraguti, Francesco
Format: Article
Language:English
Subjects:
Animals
Automatic Data Processing
Base Sequence
chromosome 7
Chromosome Mapping
Chromosomes, Human, Pair 7
Data Interpretation, Statistical
FISH
gene
Genomic Library
Humans
In Situ Hybridization, Fluorescence
mGluR3
Mice
Molecular Sequence Data
Nucleic Acid Amplification Techniques
Promoter Regions, Genetic
Receptors, Metabotropic Glutamate - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
transcription initiation
Transcription, Genetic
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Summary:In this study, the genomic organization of the human metabotropic glutamate receptor subtype 3 (mGluR3) gene has been determined. We have identified two transcription initiation sites and the polyadenylation signal by using 5′-rapid amplification of cDNA ends (RACE) and 3′-RACE, respectively. The exon/intron organization of the human mGluR3 gene revealed the presence of 6 exons separated by 5 introns. The size of introns varied from 10.4 to 120 kbp that contained consensus sequences for repetitive elements such as Alu and long interspersed elements. A putative promoter region flanking the 5′ sequence of exon 1 was identified by computer-aided analysis. The putative promoter region was characterized by the presence of a CAAT and GC box, and the absence of a TATA box or CpG islands. Several putative binding sites for transcription factors were also identified. In addition, we have isolated, from a mouse genomic library, part of the mouse mGluR3 gene and found it to correspond to exon 2 in the human mGluR3 gene. The mouse mGluR3 gene was then mapped by fluorescent in situ hybridization analysis to chromosome 5qA2.
ISSN:0167-7063
1563-5260
DOI:10.3109/01677060009084499