Multiple Potential Regulatory Elements in the 5′ Flanking Region of the Human al,-Adrenergic Receptor:Short Communication

In spite of their critical importance in myocardial hypertrophy and benign prostatic hyperplasia, nothing is known about mechanisms underlying transcriptional regulation of αla-adrenergic receptors (αl, ARs). Therefore we cloned 6.2kb of novel sequence upstream of the initiator ATG in the human αlaA...

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Bibliographic Details
Published in:DNA sequence 1998, Vol.8 (4), p.271-276
Main Authors: Lee, Keesoo, Richardson, Charlene D., Razik, Mona A., Kwatra, Madan M., Schwinn, Debra A.
Format: Article
Language:English
Subjects:
5′ flanking region
a-adrenergic
adrenergic
cis regulatory element
gene
Receptor
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Summary:In spite of their critical importance in myocardial hypertrophy and benign prostatic hyperplasia, nothing is known about mechanisms underlying transcriptional regulation of αla-adrenergic receptors (αl, ARs). Therefore we cloned 6.2kb of novel sequence upstream of the initiator ATG in the human αlaAR gene. Sequence analysis reveals a TATA-less promoter, the presence of several initiator (Inr) consensus sequences, multiple GC rich regions consistent with Sp-1 binding, and consensus sequences for AP-1 and AP-2 as well as putative cis transcriptional regulatory elements for binding of CREB (cyclic-AMP response element binding protein), glucocorticoids, estrogen, and insulin. Compared to the αlbAR, the αlaAR has several more cis regulatory elements, suggesting more complex regulation. The importance of αlaARs in human disease makes it imperative to determine mechanisms underlying transcription and ultimately expression of this receptor. These studies can now be undertaken with the availability of human αlaAR 5′-flanking and 5′-untranslated sequence.
ISSN:1042-5179
1029-2365
DOI:10.3109/10425179809008464