Multicomponent Investigations of the Hydrogen Peroxide- and Hydroxyl Radical-Scavenging Antioxidant Capacities of Biofluids: The Roles of Endogenous Pyruvate and Lactate Relevance to Inflammatory Joint Diseases

• Published in: Free radical research 1997, Vol.26 (1), p.19-35
• Main Authors: Herz, Herman, Blake, David R., Grootveld, Martin
• Format: Article
• Language: English
• Subjects: Adult; Aged; Arthritis - metabolism; Female; Free Radical Scavengers - chemistry; Free Radical Scavengers - metabolism; Humans; Hydrogen Peroxide - blood; Hydrogen Peroxide - chemistry; Hydrogen Peroxide - pharmacology; Hydroxyl Radical - chemistry; Hydroxyl Radical - metabolism; Knee Joint; Lactic Acid - analysis; Lactic Acid - chemistry; Lactic Acid - metabolism; Magnetic Resonance Spectroscopy; Male; Middle Aged; Pyruvic Acid - analysis; Pyruvic Acid - chemistry; Pyruvic Acid - metabolism; Synovial Fluid - metabolism
• ISSN: 1071-5762; 1029-2470
• DOI: 10.3109/10715769709097781

High field proton (1H) nuclear magnetic resonance (NMR) analysis of biofluids (healthy human blood sera and inflammatory knee-joint synovial fluids) has been employed to evaluate the hydrogen peroxide (H2O2)- and hydroxyl radical (°OH)- scavenging antioxidant capacities of a range of polar, low-molecular-mass endogenous metabolites therein. Data obtained indicate that consumption of H2O2 by pyruvate (generating acetate and CO2 via an oxidative decarboxylation reaction) and °OH radical by lactate (generating pyruvate, and subs quently acetate and CO2) may serve to protect alternative biofluid components (e.g., macromolecules) against reactive oxygen species-mediated oxidative damage in vivo. The mechanistic, physiological and potential therapeutic implications of these results are discussed with special reference to inflammatory joint diseases.