Prevention of Intracellular Oxidative Stress to Lens by Pyruvate and Its Ester

Pyruvate is a well-known scavenger of hydrogen peroxide (H2O2). In addition, it scavenges superoxide radical (O2). However, evidence on its intracellular antioxi-dant function is meager at present. Hence, we have examined the effectivekiess of this metabolite and its ethyl ester against intracellula...

Full description

Saved in:
Bibliographic Details
Published in:Free radical research 1998-01, Vol.28 (2), p.131-135
Main Authors: Varma, S. D., Devamanoharan, P. S., Ali, A. H.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Animals
Antioxidants - pharmacology
cataract
Energy Metabolism - drug effects
eye lens
Free Radical Scavengers - pharmacology
Ion Transport - drug effects
Lens, Crystalline - drug effects
Lens, Crystalline - metabolism
menadione
Oxidation-Reduction
Oxidative Stress - drug effects
Oxygen radicals
pyruvate
pyruvate-ester
Pyruvates - pharmacology
Pyruvic Acid - pharmacology
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species - metabolism
Rubidium - metabolism
Vitamin K - pharmacology
Vitamin K - toxicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pyruvate is a well-known scavenger of hydrogen peroxide (H2O2). In addition, it scavenges superoxide radical (O2). However, evidence on its intracellular antioxi-dant function is meager at present. Hence, we have examined the effectivekiess of this metabolite and its ethyl ester against intracellular oxidative damage to the lens under organ culture. Menadione, a redoxcycling quinone, was used to generate the reactive oxygen species (ROS). It was found to inhibit lens metabolism as evidenced by a decrease of ATP. Additionally, tissue oxidation was apparent by loss of glutathione (GSH), and increase in the level of oxidized glutathione (GSSG), coupled with increase of the urea soluble proteins (water insoluble). The overall physiological damage was apparent by the inhibition of the Na+-K+-ATPase dependent cation pump, as evidenced by a decreased rubidium transport. These deleterious effects were attenuated by pyruvate and ethyl-pyruvate. The later was found to be more effective.
ISSN:1071-5762
1029-2470
DOI:10.3109/10715769809065799