Measurement of Lipid Peroxidation

Lipid peroxidation results in the formation of conjugated dienes, lipid hydroperoxides and degradation products such as alkanes, aldehydes and isoprostanes. The approach to the quantitative assessment of lipid peroxidation depends on whether the samples involve complex biological material obtained i...

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Bibliographic Details
Published in:Free radical research 1998-01, Vol.28 (6), p.659-671
Main Authors: Moore, Kevin, Roberts, L. Jackson
Format: Article
Language:English
Subjects:
Aldehydes - analysis
Animals
Biochemistry - methods
Chromatography, High Pressure Liquid
Fluorescence
Humans
Lipid Peroxidation
Malondialdehyde - analysis
Malondialdehyde - metabolism
Mass Spectrometry
Oxidative Stress
Prostaglandins - analysis
Prostaglandins - chemistry
Thiobarbituric Acid Reactive Substances - analysis
Thiobarbituric Acid Reactive Substances - chemistry
Thiobarbituric Acid Reactive Substances - metabolism
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Summary:Lipid peroxidation results in the formation of conjugated dienes, lipid hydroperoxides and degradation products such as alkanes, aldehydes and isoprostanes. The approach to the quantitative assessment of lipid peroxidation depends on whether the samples involve complex biological material obtained in vivo, or whether the samples involve relatively simple mixtures obtained in vituo. Samples obtained in vivo contain a large number of products which themselves may undergo metabolism. The measurement of conjugated diene formation is generally applied as a dynamic quantitation e.g. during the oxidation of LDL, and is not generally applied to samples obtained in vivo. Lipid hydroperoxides readily decompose, but can be measured directly and indirectly by a variety of techniques. The measurement of MDA by the TBAR assay is non-specific, and is generally poor when applied to biological samples. More recent assays based on the measurement of MDA or HNE-lysine adducts are likely to be more applicable to biological samples, since adducts of these reactive aldehydes are relatively stable. The discovery of the isoprostanes as lipid peroxidation products which can be measured by gas chromatography mass spectrometry or immunoassay has opened a new avenue by which to quantify lipid peroxidation in vivo, and will be discussed in detail.
ISSN:1071-5762
1029-2470
DOI:10.3109/10715769809065821