Evaluation of 99mTc-MAMA-chrysamine G as an in vivo probe for amyloidosis

To date, systemic amyloidosis is diagnosed histologi-cally using Congo red staining or in vivo using iodine-123 labelled serum amyloid P component (1231-SAP) scintigra-phy. We developed 99mTc-MAMA-CG, a 99mTc-labelled derivative of the lipophilic Congo red analogue chtysamine G (CG), as a possible a...

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Bibliographic Details
Published in:Amyloid 2001-09, Vol.8 (3), p.202-214
Main Authors: Dezutter, Nancy A., Landman, Wil J.M, Jager, Pieter L., Groot, Tjibbe J. de, Dupont, Patrick J., Tooten, Peter C.J., Zekarias, Bereket, Gruys, Erik, Verbruggen, Alfons M.
Format: Article
Language:English
Subjects:
amyloid
Amyloid - analysis
Amyloidosis - diagnosis
Amyloidosis - diagnostic imaging
Amyloidosis - microbiology
Animals
Autoradiography - methods
Benzoates - chemistry
Chickens
Chrysamine G
Congo red
Disease Models, Animal
Enterococcus faecalis - pathogenicity
Evaluation Studies as Topic
Female
I-SAP
in vivo probe
Iodine Radioisotopes - chemistry
Molecular Probes
Organotechnetium Compounds - chemistry
Radionuclide Imaging
Serum Amyloid P-Component
SPECT
technetium-99m
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Summary:To date, systemic amyloidosis is diagnosed histologi-cally using Congo red staining or in vivo using iodine-123 labelled serum amyloid P component (1231-SAP) scintigra-phy. We developed 99mTc-MAMA-CG, a 99mTc-labelled derivative of the lipophilic Congo red analogue chtysamine G (CG), as a possible alternative to 1231-SAP. In vivo 99mTc-MAMA-CG scintigraphy, performed in chickens with spontaneous joint amyloidosis, resulted as soon as 10 min after injection in scintigraphic images showing uptake of activity in amyloid-loaded organs (liver, joints). One of these chickens was studied also with 123I-SAP resulting in scintigraphic images revealing 1231-SAP binding to amyloid deposits in the liver. However, up to II h after injection no radioactivity was visible in the amyloid positive joints. In vitro autoradiography, performed on sections of chicken joints with Enterococcus faecalis induced amyloid arthropathy (chjAA), demonstrated the failure of 99mTc-MAMA-CG to bind significantly to amyloid deposits in the presence of 10M Congo red. The speclficity of 99mTc-MAMA-CG localisation was also established by the absence of 99mTc-MAMA-CG binding in non-amyloidotic organs in vitro and in vivo. 99mTc-MAMA-CG did not show any sign of acute toxicity. These findings establish the usefulness of 99mTc-MAMA-CG as a non-invusive in vivo diagnostic probe in chickens with amyloid arthropathy and suggest that it may also be applicable to human amyloidosis.
ISSN:1350-6129
1744-2818
DOI:10.3109/13506120109007363