Intracellular localization and physiological function of a rice Ca2+-permeable channel OsTPC1

Two-pore channels (TPCs) are cation channels with a voltage-sensor domain conserved in plants and animals. Rice OsTPC1 is predominantly localized to the plasma membrane (PM), and assumed to play an important role as a Ca 2+ -permeable cation channel in the regulation of cytosolic Ca 2+ rise and inna...

Full description

Saved in:
Bibliographic Details
Published in:Plant Signaling & Behavior 2012, Vol.7 (11), p.1428-1430
Main Authors: Kurusu, Takamitsu, Hamada, Haruyasu, Koyano, Tomoko, Kuchitsu, Kazuyuki
Format: Report
Language:English
Subjects:
channel
intracellular localization
plant cells
two-pore channel 1
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two-pore channels (TPCs) are cation channels with a voltage-sensor domain conserved in plants and animals. Rice OsTPC1 is predominantly localized to the plasma membrane (PM), and assumed to play an important role as a Ca 2+ -permeable cation channel in the regulation of cytosolic Ca 2+ rise and innate immune responses including hypersensitive cell death and phytoalexin biosynthesis in cultured rice cells triggered by a fungal elicitor, xylanase from Trichoderma viride. In contrast, Arabidopsis AtTPC1 is localized to the vacuolar membrane (VM). To gain further insights into the intracellular localization of OsTPC1, we stably expressed OsTPC1-GFP in tobacco BY-2 cells. Confocal imaging and membrane fractionation revealed that, unlike in rice cells, the majority of OsTPC1-GFP fusion protein was targeted to the VM in tobacco BY-2 cells. Intracellular localization and functions of the plant TPC family is discussed.
ISSN:1559-2324
DOI:10.4161/psb.22086