Transillumination lab-on-a-chip cytometer with silicon/glass membrane for image-based porcine oocyte deformation characterisation

Transillumination microscopes, often with a simple lens-free optical configuration, combined with lab-on-a-chip devices are useful tools for the characterisation of various biological samples. A key issue with these devices is light transparency across a lab-on-a-chip structure. In this work we achi...

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Bibliographic Details
Published in:Journal of micromechanics and microengineering 2024-01, Vol.34 (1), p.17001
Main Authors: Pokrzywnicka, Aleksandra, Lizanets, Danylo, Walczak, Rafał
Format: Article
Language:English
Subjects:
glass
MEMS cytometer
transillumination imagining
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Summary:Transillumination microscopes, often with a simple lens-free optical configuration, combined with lab-on-a-chip devices are useful tools for the characterisation of various biological samples. A key issue with these devices is light transparency across a lab-on-a-chip structure. In this work we achieved this by embedding a glass window in a silicon membrane. Despite light transmission, the membrane could be pressure actuated. A second key issue is software analysis of the images due to the holographic nature of the captured images. In this paper, the technology of the silicon/glass membrane and results of porcine oocyte imaging during deformation are presented and compared with our previous micro-electro-mechanical system cytometer working with a reflective microscope. Thus, a unique device that deforms cells and allows deformation measurements with transillumination was developed.
ISSN:0960-1317
1361-6439
DOI:10.1088/1361-6439/ad0d81