Transillumination lab-on-a-chip cytometer with silicon/glass membrane for image-based porcine oocyte deformation characterisation

Transillumination microscopes, often with a simple lens-free optical configuration, combined with lab-on-a-chip devices are useful tools for the characterisation of various biological samples. A key issue with these devices is light transparency across a lab-on-a-chip structure. In this work we achi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of micromechanics and microengineering 2024-01, Vol.34 (1), p.17001
Main Authors: Pokrzywnicka, Aleksandra, Lizanets, Danylo, Walczak, Rafał
Format: Article
Language:English
Subjects:
glass
MEMS cytometer
transillumination imagining
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c307t-4a33323de4e17ce1ae777c34bd3a62e6875fd54362698ea579240b77c3bf8b93
container_end_page
container_issue 1
container_start_page 17001
container_title Journal of micromechanics and microengineering
container_volume 34
creator Pokrzywnicka, Aleksandra
Lizanets, Danylo
Walczak, Rafał
description Transillumination microscopes, often with a simple lens-free optical configuration, combined with lab-on-a-chip devices are useful tools for the characterisation of various biological samples. A key issue with these devices is light transparency across a lab-on-a-chip structure. In this work we achieved this by embedding a glass window in a silicon membrane. Despite light transmission, the membrane could be pressure actuated. A second key issue is software analysis of the images due to the holographic nature of the captured images. In this paper, the technology of the silicon/glass membrane and results of porcine oocyte imaging during deformation are presented and compared with our previous micro-electro-mechanical system cytometer working with a reflective microscope. Thus, a unique device that deforms cells and allows deformation measurements with transillumination was developed.
doi_str_mv 10.1088/1361-6439/ad0d81
format article
fullrecord <record><control><sourceid>iop_cross</sourceid><recordid>TN_cdi_iop_journals_10_1088_1361_6439_ad0d81</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>jmmad0d81</sourcerecordid><originalsourceid>FETCH-LOGICAL-c307t-4a33323de4e17ce1ae777c34bd3a62e6875fd54362698ea579240b77c3bf8b93</originalsourceid><addsrcrecordid>eNp1kM9LwzAYhoMoOKd3j7l5MS5puiY9yvAXDLzsHtLk65bRNCXpkB39z82seNLTBy_P-_LxIHTL6AOjUi4YrxipSl4vtKVWsjM0-43O0YzWFSWMM3GJrlLaU8qYZHKGPjdR98l13cG7Xo8u9LjTDQk90cTs3IDNcQweRoj4w407nFFnQr_Ydjol7ME3uQ-4DRE7r7dAGp3A4iFE43IeQu4DtpABP82bnY7a5EGXvoNrdNHqLsHNz52jzfPTZvVK1u8vb6vHNTGcipGUmnNecAslMGGAaRBCGF42luuqgEqKZWuXJa-Kqpagl6IuStqckKaVTc3niE6zJoaUIrRqiPnjeFSMqpNBddKlTrrUZDBX7qaKC4Pah0Ps839q773ipWKKMpE1qsG2mbz_g_x3-Avu1ILi</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Transillumination lab-on-a-chip cytometer with silicon/glass membrane for image-based porcine oocyte deformation characterisation</title><source>Institute of Physics:Jisc Collections:IOP Publishing Read and Publish 2024-2025 (Reading List)</source><creator>Pokrzywnicka, Aleksandra ; Lizanets, Danylo ; Walczak, Rafał</creator><creatorcontrib>Pokrzywnicka, Aleksandra ; Lizanets, Danylo ; Walczak, Rafał</creatorcontrib><description>Transillumination microscopes, often with a simple lens-free optical configuration, combined with lab-on-a-chip devices are useful tools for the characterisation of various biological samples. A key issue with these devices is light transparency across a lab-on-a-chip structure. In this work we achieved this by embedding a glass window in a silicon membrane. Despite light transmission, the membrane could be pressure actuated. A second key issue is software analysis of the images due to the holographic nature of the captured images. In this paper, the technology of the silicon/glass membrane and results of porcine oocyte imaging during deformation are presented and compared with our previous micro-electro-mechanical system cytometer working with a reflective microscope. Thus, a unique device that deforms cells and allows deformation measurements with transillumination was developed.</description><identifier>ISSN: 0960-1317</identifier><identifier>EISSN: 1361-6439</identifier><identifier>DOI: 10.1088/1361-6439/ad0d81</identifier><identifier>CODEN: JMMIEZ</identifier><language>eng</language><publisher>IOP Publishing</publisher><subject>glass ; MEMS cytometer ; transillumination imagining</subject><ispartof>Journal of micromechanics and microengineering, 2024-01, Vol.34 (1), p.17001</ispartof><rights>2023 The Author(s). Published by IOP Publishing Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c307t-4a33323de4e17ce1ae777c34bd3a62e6875fd54362698ea579240b77c3bf8b93</cites><orcidid>0000-0002-9371-9414 ; 0000-0002-9903-6390</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Pokrzywnicka, Aleksandra</creatorcontrib><creatorcontrib>Lizanets, Danylo</creatorcontrib><creatorcontrib>Walczak, Rafał</creatorcontrib><title>Transillumination lab-on-a-chip cytometer with silicon/glass membrane for image-based porcine oocyte deformation characterisation</title><title>Journal of micromechanics and microengineering</title><addtitle>JMM</addtitle><addtitle>J. Micromech. Microeng</addtitle><description>Transillumination microscopes, often with a simple lens-free optical configuration, combined with lab-on-a-chip devices are useful tools for the characterisation of various biological samples. A key issue with these devices is light transparency across a lab-on-a-chip structure. In this work we achieved this by embedding a glass window in a silicon membrane. Despite light transmission, the membrane could be pressure actuated. A second key issue is software analysis of the images due to the holographic nature of the captured images. In this paper, the technology of the silicon/glass membrane and results of porcine oocyte imaging during deformation are presented and compared with our previous micro-electro-mechanical system cytometer working with a reflective microscope. Thus, a unique device that deforms cells and allows deformation measurements with transillumination was developed.</description><subject>glass</subject><subject>MEMS cytometer</subject><subject>transillumination imagining</subject><issn>0960-1317</issn><issn>1361-6439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp1kM9LwzAYhoMoOKd3j7l5MS5puiY9yvAXDLzsHtLk65bRNCXpkB39z82seNLTBy_P-_LxIHTL6AOjUi4YrxipSl4vtKVWsjM0-43O0YzWFSWMM3GJrlLaU8qYZHKGPjdR98l13cG7Xo8u9LjTDQk90cTs3IDNcQweRoj4w407nFFnQr_Ydjol7ME3uQ-4DRE7r7dAGp3A4iFE43IeQu4DtpABP82bnY7a5EGXvoNrdNHqLsHNz52jzfPTZvVK1u8vb6vHNTGcipGUmnNecAslMGGAaRBCGF42luuqgEqKZWuXJa-Kqpagl6IuStqckKaVTc3niE6zJoaUIrRqiPnjeFSMqpNBddKlTrrUZDBX7qaKC4Pah0Ps839q773ipWKKMpE1qsG2mbz_g_x3-Avu1ILi</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Pokrzywnicka, Aleksandra</creator><creator>Lizanets, Danylo</creator><creator>Walczak, Rafał</creator><general>IOP Publishing</general><scope>O3W</scope><scope>TSCCA</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-9371-9414</orcidid><orcidid>https://orcid.org/0000-0002-9903-6390</orcidid></search><sort><creationdate>20240101</creationdate><title>Transillumination lab-on-a-chip cytometer with silicon/glass membrane for image-based porcine oocyte deformation characterisation</title><author>Pokrzywnicka, Aleksandra ; Lizanets, Danylo ; Walczak, Rafał</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c307t-4a33323de4e17ce1ae777c34bd3a62e6875fd54362698ea579240b77c3bf8b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>glass</topic><topic>MEMS cytometer</topic><topic>transillumination imagining</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pokrzywnicka, Aleksandra</creatorcontrib><creatorcontrib>Lizanets, Danylo</creatorcontrib><creatorcontrib>Walczak, Rafał</creatorcontrib><collection>Open Access: IOP Publishing Free Content</collection><collection>IOPscience (Open Access)</collection><collection>CrossRef</collection><jtitle>Journal of micromechanics and microengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pokrzywnicka, Aleksandra</au><au>Lizanets, Danylo</au><au>Walczak, Rafał</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transillumination lab-on-a-chip cytometer with silicon/glass membrane for image-based porcine oocyte deformation characterisation</atitle><jtitle>Journal of micromechanics and microengineering</jtitle><stitle>JMM</stitle><addtitle>J. Micromech. Microeng</addtitle><date>2024-01-01</date><risdate>2024</risdate><volume>34</volume><issue>1</issue><spage>17001</spage><pages>17001-</pages><issn>0960-1317</issn><eissn>1361-6439</eissn><coden>JMMIEZ</coden><abstract>Transillumination microscopes, often with a simple lens-free optical configuration, combined with lab-on-a-chip devices are useful tools for the characterisation of various biological samples. A key issue with these devices is light transparency across a lab-on-a-chip structure. In this work we achieved this by embedding a glass window in a silicon membrane. Despite light transmission, the membrane could be pressure actuated. A second key issue is software analysis of the images due to the holographic nature of the captured images. In this paper, the technology of the silicon/glass membrane and results of porcine oocyte imaging during deformation are presented and compared with our previous micro-electro-mechanical system cytometer working with a reflective microscope. Thus, a unique device that deforms cells and allows deformation measurements with transillumination was developed.</abstract><pub>IOP Publishing</pub><doi>10.1088/1361-6439/ad0d81</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-9371-9414</orcidid><orcidid>https://orcid.org/0000-0002-9903-6390</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0960-1317
ispartof Journal of micromechanics and microengineering, 2024-01, Vol.34 (1), p.17001
issn 0960-1317
1361-6439
language eng
recordid cdi_iop_journals_10_1088_1361_6439_ad0d81
source Institute of Physics:Jisc Collections:IOP Publishing Read and Publish 2024-2025 (Reading List)
subjects glass
MEMS cytometer
transillumination imagining
title Transillumination lab-on-a-chip cytometer with silicon/glass membrane for image-based porcine oocyte deformation characterisation
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T14%3A35%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-iop_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transillumination%20lab-on-a-chip%20cytometer%20with%20silicon/glass%20membrane%20for%20image-based%20porcine%20oocyte%20deformation%20characterisation&rft.jtitle=Journal%20of%20micromechanics%20and%20microengineering&rft.au=Pokrzywnicka,%20Aleksandra&rft.date=2024-01-01&rft.volume=34&rft.issue=1&rft.spage=17001&rft.pages=17001-&rft.issn=0960-1317&rft.eissn=1361-6439&rft.coden=JMMIEZ&rft_id=info:doi/10.1088/1361-6439/ad0d81&rft_dat=%3Ciop_cross%3Ejmmad0d81%3C/iop_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c307t-4a33323de4e17ce1ae777c34bd3a62e6875fd54362698ea579240b77c3bf8b93%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true