In vitro anti-oxidant and cytotoxic activities of gold nanoparticles synthesized from an aqueous extract of the Xylopia aethiopica fruit

The development of gold nanoparticles (AuNPs) using green approach has drawn a considerable interest in the field of nanomedicine. Its wide application in clinical diagnosis, imaging and therapeutics portrays its importance for human existence. In this study, we reported the biogenic synthesis of Au...

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Bibliographic Details
Published in:Nanotechnology 2021-07, Vol.32 (31), p.315101
Main Authors: Anadozie, Scholastica O, Adewale, Olusola B, Meyer, Mervin, Davids, Hajierah, Roux, Saartjie
Format: Article
Language:English
Subjects:
anti-oxidant
biogenic
cytotoxic
gold nanoparticles
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Summary:The development of gold nanoparticles (AuNPs) using green approach has drawn a considerable interest in the field of nanomedicine. Its wide application in clinical diagnosis, imaging and therapeutics portrays its importance for human existence. In this study, we reported the biogenic synthesis of AuNPs using aqueous extract of Xylopia aethiopica fruit (AEXAf), which act as both reducing and stabilizing agent. The characterization of AEXAf-AuNPs was performed using ultraviolet visible spectroscopy, dynamic light scattering and zeta potential measurements, high-resolution transmission electron microscopy and Fourier transform-infrared spectroscopy. In vitro anti-oxidant activities of the AEXAf-AuNPs and AEXAf were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP). The in vitro cytotoxic activities of AEXAf-AuNPs and AEXAf against breast and colorectal cancer cells were evaluated using 3,-(4,5 dimethylthiazol)-2,5 diphenyl tetrazolium bromide (MTT) viability and annexin-V/PI assays. The AEXAf-AuNPs exhibited surface plasmon absorption maximum at 522 nm and were stabled for 4 weeks. The average size of AEXAf-AuNPs was 10.61±3.33 nm on the high-resolution transmission electron microscopy images. The in vitro anti-oxidant activities of AEXAf-AuNPs and AEXAf were concentration-dependent. The AEXAf-AuNPs was cytotoxic to the cancer cells and non-toxic to the non-cancerous human fibroblast cells (KMST-6) (up to 200 µg/mL). From these results, AEXAf-AuNPs showed good anti-oxidant and anti-cancer activities, and can be suggested as possible therapeutic agent for breast and colorectal cancers.
ISSN:0957-4484
1361-6528
DOI:10.1088/1361-6528/abf6ee