Photodynamic therapy using silicon phthalocyanine conjugated with bovine serum albumin as a drug delivery system

In the present study, we describe a new silicon phthalocyanine conjugated to bovine serum albumin (PcSiN3M-BSA) and its photodynamic activity in murine macrophages cells (J774.A1). The nonconjugated precursor, bis(trimethylaminoethanoxy)–phthalocyaninato silicon (IV) (PcSiN3M), was also studied. Com...

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Bibliographic Details
Published in:Laser physics 2021-06, Vol.31 (7), p.75601
Main Authors: Silva, E P O, Ribeiro, N M, Cardoso, M A G, Pacheco-Soares, C, Jr, M Beltrame
Format: Article
Language:English
Subjects:
bovine serum albumin (BSA)
murine macrophages cells (J774.A1)
photodynamic therapy (PDT)
phthalocyanines
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Summary:In the present study, we describe a new silicon phthalocyanine conjugated to bovine serum albumin (PcSiN3M-BSA) and its photodynamic activity in murine macrophages cells (J774.A1). The nonconjugated precursor, bis(trimethylaminoethanoxy)–phthalocyaninato silicon (IV) (PcSiN3M), was also studied. Compounds PcSiN3M and PcSiN3M-BSA showed no cytotoxicity in the dark, but exhibited high photodynamic activities following exposure to 5 µ M photosensitizers and 45 J cm −2 irradiation. These conditions were sufficient to decrease the cell viability to 40% and 5% in cells treated with PcSiN3M and PcSiN3M-BSA, respectively. These results demonstrated an increase of 87% in the photodynamic activity of PcSiN3M when conjugated with BSA. The results shown in this work suggest that PcSiN3M-BSA had higher uptake by J774.A1 cells, which contributed to its higher photoactivity compared with the unconjugated form, PcSiN3N.
ISSN:1054-660X
1555-6611
DOI:10.1088/1555-6611/abfe58