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Optical trapping and arrangement with reconfigurable "bottle" beam for digital holographic microscopy

The design and construction of optical tweezers based on uniaxial crystal anisotropy for generation of adjustable "bottle" beam trap carrying optical vortex with orbital angular momentum is considered. In coupling with digital holographic microscopy, optical trapping becomes a high precisi...

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Bibliographic Details
Published in:Journal of physics. Conference series 2019-12, Vol.1410 (1), p.12162
Main Authors: Shostka, N V, Sokolenko, B N, Karakcheva, O S, Poletaev, D A, Titova, A O, Prisyazhniuk, A V, Ismailov, I A
Format: Article
Language:English
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Summary:The design and construction of optical tweezers based on uniaxial crystal anisotropy for generation of adjustable "bottle" beam trap carrying optical vortex with orbital angular momentum is considered. In coupling with digital holographic microscopy, optical trapping becomes a high precision instrument for visualization, shape definition and refractivity measurements of isolated micro structures and biological objects in-situ. The non-destructive and sterile non-contact tweezing of specimens or their parts in localized intensity minima of coherent vortex beam was performed with using of 200 mW semiconductor 532 nm trapping laser and LiNbO3 crystal. Visualization and position control of trapped marine centric diatoms was performed by a lens-free axial digital holographic microscopy in liquid medium.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/1410/1/012162