Automation of Silica Bead-based Nucleic Acid Extraction on a Centrifugal Lab-on-a-Disc Platform

We describe a centrifugal microfluidic 'Lab-on-a-Disc' (LoaD) technology for DNA purification towards eventual integration into a Sample-to-Answer platform for detection of the pathogen Escherichia coli O157:H7 from food samples. For this application, we use a novel microfluidic architectu...

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Bibliographic Details
Published in:Journal of physics. Conference series 2016-10, Vol.757 (1), p.12013
Main Authors: Kinahan, David J, Mangwanya, Faith, Garvey, Robert, Chung, Danielle WY, Lipinski, Artur, Julius, Lourdes AN, King, Damien, Mohammadi, Mehdi, Mishra, Rohit, Al-Ofi, May, Miyazaki, Celina, Ducrée, Jens
Format: Article
Language:English
Subjects:
E coli
Flow control
Fluorescence
Microfluidics
Nucleic acids
Optimization
Physics
Silicon dioxide
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Summary:We describe a centrifugal microfluidic 'Lab-on-a-Disc' (LoaD) technology for DNA purification towards eventual integration into a Sample-to-Answer platform for detection of the pathogen Escherichia coli O157:H7 from food samples. For this application, we use a novel microfluidic architecture which combines 'event-triggered' dissolvable film (DF) valves with a reaction chamber gated by a centrifugo-pneumatic siphon valve (CPSV). This architecture permits comprehensive flow control by simple changes in the speed of the platform innate spindle motor. Even before method optimisation, characterisation by DNA fluorescence reveals an extraction efficiency of 58%, which is close to commercial spin columns.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/757/1/012013