Callus induction and flavonoid production on the immature seed of Stelechocarpus burahol

Stelechocarpus burahol [(Bl.) Hook. f. & Th.] is one of the medicinal plants. In vitro callus induction studies on S. burahol were carried out to determine phytohormone requirement for optimum callus induction. Immature seed explants were cultured on MS medium by adding different kinds and diffe...

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Bibliographic Details
Published in:Journal of physics. Conference series 2018-03, Vol.983 (1), p.12186
Main Authors: Habibah, N A, Moeljopawiro, S, Dewi, K, Indrianto, A
Format: Article
Language:English
Subjects:
callus
flavonoid
Flavonoids
Growth regulators
Herbal medicine
Physics
Seed
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Summary:Stelechocarpus burahol [(Bl.) Hook. f. & Th.] is one of the medicinal plants. In vitro callus induction studies on S. burahol were carried out to determine phytohormone requirement for optimum callus induction. Immature seed explants were cultured on MS medium by adding different kinds and different concentrations of plant growth regulators (picloram and 2,4-D) under light and dark conditions. The results showed that callus formation was initiated on the 18,50th to the 55th days. The best condition for optimum callus induction was found on MS medium, which was supplemented with 7.5 mg/L picloram and was maintained in the dark condition. The callus induction varied from 60% to 100%. The callus that produced the highest flavonoid was grown on the medium with the addition of 10 mg/L of 2,4-D. In conclusion, the results represented a suitable medium for S.burahol callus induction.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/983/1/012186