Peptide modified polymer poly (glycerol- dodecanedioate co-fumarate) for efficient control of motor neuron differentiation

Neural tissue engineering is one of the most promising approaches for healing nerve damage, which bypasses the limits of contemporary conventional treatments. In a previous study, we developed a fibrous scaffold via electrospinning poly (glycerol dodecanedioate) (PGD) and gelatin that mimics the str...

Full description

Saved in:
Bibliographic Details
Published in:Biomedical materials (Bristol) 2015-11, Vol.10 (6), p.065013-065013
Main Authors: Dai, Xizi, Huang, Yen-Chih, Leichner, Jared, Nair, Madhvan, Lin, Wei-Chiang, Li, Chen-Zhong
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion - physiology
Cell Differentiation - physiology
Cell Proliferation - physiology
Cells, Cultured
Differentiation
electrospinning
Equipment Design
Equipment Failure Analysis
Fibers
Fumarates - chemistry
fumaric acid
Glycerols
Laminin
Materials Testing
Mice
motor neuron
Motor Neurons - cytology
Motor Neurons - physiology
Motors
Neural Stem Cells - cytology
Neural Stem Cells - physiology
Neurogenesis - physiology
Oligopeptides - pharmacokinetics
peptide
Peptides
poly (glycerol- dodecanedioate co-fumarate)
Polyesters - chemistry
Scaffolds
stem cell
Tissue engineering
Tissue Scaffolds
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Neural tissue engineering is one of the most promising approaches for healing nerve damage, which bypasses the limits of contemporary conventional treatments. In a previous study, we developed a fibrous scaffold via electrospinning poly (glycerol dodecanedioate) (PGD) and gelatin that mimics the structure of a native extracellular matrix (ECM) for soft tissue engineering application. In this study, fumaric acid (FA) was incorporated into the PGD synthesis process, which produced a PGD derivative referred to as poly (glycerol dodecanedioate co-fumarate) (PGDF). This introduced a new functional group, a double bond, into the polymer thus providing new modification possibilities. Arg-Gly-Asp-Cys (RGDC) and laminin peptides were chosen as biomolecules to modify the fiber and facilitate cell attachment and differentiation efficiency. The release of FA into the medium was quantified to investigate the bioreactivity of the derived scaffolds. In combination with UV crosslinking, the developed PGDF fiber mats were able to withstand degradation processes for up to 2 months, which ensures that neural tissue engineering applications are viable. Cell viability and motor neuron differentiation efficiency were demonstrated to be significantly improved with the addition of FA, RGDC and laminin peptides.
ISSN:1748-6041
1748-605X
1748-605X
DOI:10.1088/1748-6041/10/6/065013