Real-time monitoring of hydrogen cyanide (HCN) and ammonia (NH3) emitted by Pseudomonas aeruginosa

We present the real-time monitoring of hydrogen cyanide (HCN) production from Pseudomonas aeruginosa (P. aeruginosa) strains in vitro, using laser-based photoacoustic spectroscopy. Simultaneously, the production of ammonia (NH3) was measured, and the influence of different factors (e.g. the medium,...

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Bibliographic Details
Published in:Journal of breath research 2015-01, Vol.9 (2), p.027102-027102
Main Authors: Neerincx, Anne H, Mandon, Julien, van Ingen, Jakko, Arslanov, Denis D, Mouton, Johan W, Harren, Frans J M, Merkus, Peter J F M, Cristescu, Simona M
Format: Article
Language:English
Subjects:
Acrylics
ammonia
Ammonia - metabolism
Colony Count, Microbial
cystic fibrosis
Cystic Fibrosis - microbiology
Humans
hydrogen cyanide
Hydrogen Cyanide - metabolism
laser-based photoacoustic spectroscopy
Microbial Sensitivity Tests
Pathogens
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - growth & development
Pseudomonas aeruginosa - isolation & purification
Pseudomonas aeruginosa - metabolism
Tobramycin - pharmacology
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Summary:We present the real-time monitoring of hydrogen cyanide (HCN) production from Pseudomonas aeruginosa (P. aeruginosa) strains in vitro, using laser-based photoacoustic spectroscopy. Simultaneously, the production of ammonia (NH3) was measured, and the influence of different factors (e.g. the medium, temperature and antibiotics treatment) was assessed. Both reference strains and clinical isolates of patients with CF were studied, and compared to other pathogens commonly present in lungs/airways of CF patients. Hydrogen cyanide production starts to rise as soon as P. aeruginosa bacteria reach the stationary phase ((9.0-9.5) × 109 colony forming units, CFUs), up to concentrations of 14.5 microliters per hour (µl h−1). Different strains of P. aeruginosa produced HCN to varying degrees, and addition of tobramycin strongly reduced HCN production within 2 h from application. Burkholderia cepacia also produced HCN (up to 0.35µl h−1 in 9.0  ×  109 CFU) while other pathogens (Aspergillus fumigatus, Stenotrophomonas maltophilia, Mycobacterium abscessus) did not produce detectable levels. Our study reveals for the first time a broad overview of the dynamics of the HCN production in vitro.
ISSN:1752-7155
1752-7163
1752-7163
DOI:10.1088/1752-7155/9/2/027102