Screening of Bacterial Isolates Producing Diacetyl and Selecting the Most Efficient Isolate

Screening number of bacterial isolates in term of their ability to produce diacetyl from five samples of different dairy products include Qargoli cheese (Q and K) from two sources, Akkawi cheese (A), Soft cheese (S) and local butter (B), were purchased from the local market of Al-Ramadi city/Anbar p...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2023-11, Vol.1259 (1), p.12079
Main Authors: Al-Hashemi, Abrar N., Al-Janabi, Nidhal M. S.
Format: Article
Language:English
Subjects:
Anaerobic conditions
Butter
Cheese
Cocci
Colonies
Dairy products
Diacetyl
High-performance liquid chromatography
Liquid chromatography
Productive bacteria
Productivity
Qualitative methods
Quantitative estimate
Reagents
Screening
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Summary:Screening number of bacterial isolates in term of their ability to produce diacetyl from five samples of different dairy products include Qargoli cheese (Q and K) from two sources, Akkawi cheese (A), Soft cheese (S) and local butter (B), were purchased from the local market of Al-Ramadi city/Anbar province, the colonies were picked up from culture medium MRS and M17 after incubation 24 to 48 hr at 37C in anaerobic conditions and examined in microscopic (rod and cocci) Single, double and Strings, gram-positive and non-spore forming, we collected 7 colonies from 21 colony, 2 isolates from cheese (K) on M17 medium were cocci, 3 isolates from cheese (Q) on MRS medium were cocci, 1 isolate from cheese (A) on M17 medium was rod ales 1 isolate from cheese (S) but on MRS medium was rod, as for butter (B) no result, were shown under the same conditions, the 7 isolates subjected to a qualitative detection of their ability production and gave a positive indicator for Voges-proskauer test and Brady’s reagent to varying degrees between them, then the production of diacetyl for 4 isolates with the highest productivity, were quantified by Westerfeld method and HPLC technique, with Westerfeld the concentrations were 171.197, 234.654, 217.617, 222.926 μg/ml for K 2 , Q 3 , K 4 and K 7 respectively at absorbance 540 nm, isolate Q 3 and K 7 were distinguished with highest productivity of diacetyl by HPLC reached 5.7 and 5.9 ppm respectively.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/1259/1/012079