The palm oil-based chlorophyll removal and the evaluation of antiaging properties on Centella asiatica ethanolic extract

Centella asiatica is an essential medicinal herb in numerous traditions. The high chlorophyll concentration of C. asiatica makes dosage form formulation challenging. On the other hand, palm oil is feasible to eliminate the chlorophyll from plant-based products. This research aim to analysis the phyt...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2024-02, Vol.1312 (1), p.12041
Main Authors: Haryanti, S, Budiarti, M, Farida, S, Dewi, A P K, Supriyati, N, Jokopriyambodo, W, Wahyono, S, Widowati, L
Format: Article
Language:English
Subjects:
Aging
asiaticoside
Centella asiatica
Chlorophyll
chlorophyll-removal
Cytotoxicity
Densitometry
Ethanol
Galactosidase
Herbal medicine
Hydrogen peroxide
Intracellular
Medicinal plants
Oxidative stress
Palm oil
Phytochemicals
Senescence
senescent
Traditions
Vegetable oils
Vero cells
β-Galactosidase
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Summary:Centella asiatica is an essential medicinal herb in numerous traditions. The high chlorophyll concentration of C. asiatica makes dosage form formulation challenging. On the other hand, palm oil is feasible to eliminate the chlorophyll from plant-based products. This research aim to analysis the phytochemical profile and in vitro anti-aging effects on chlorophyll removal of C. asiatica extract. The extract was soxhletated in 70% ethanol. The extract was partitioned with 50% ethanol and palm oil to create a dechlorophyllation extract. Asiaticoside was analyzed using TLC-densitometry. The cytoprotective effect of H 2 O 2 -induced oxidative stress in Vero cells was assessed using MTT assays. Flow cytometer analysis was used for quantifying the intracellular ROS. The senescence-associated-β-galactosidase assay was used to identify senescent cells. The efficiency of chlorophyll removal by palm oil was 90.94±0.62%. The dechlorophyllation extract (DE) showed a concentration of 1.58±0.02% for asiaticoside, but it was not found in the original extract (OE). DE significantly reduced H 2 O 2 -induced cytotoxicity in Vero cells compared to OE. Both DE and OE reduced intracellular ROS and senescent cells. Palm oil-based chlorophyll removal improved the phytochemical content and anti-aging effects of C. asiatica in ethanolic extract.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/1312/1/012041