Visual Method for Detecting Contaminant on Dried Nutmeg Using Fluorescence Imaging

Traditional practice of nutmeg sun-drying causes some fungi such as Aspergillus flavus to grow. One of the secondary metabolites of A. flavus named aflatoxin (AFs) is known to be carcinogenic, so the dried nutmeg kernel must be aflatoxin-free in the trading. Aflatoxin detection requires time and cos...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2018-05, Vol.147 (1), p.12003
Main Authors: Dahlan, S A, Ahmad, U, Subrata, I D M
Format: Article
Language:English
Subjects:
Aflatoxins
Carcinogens
CCD cameras
Contaminants
Drying
Farmers
Fluorescence
Fungi
Kernels
Light sources
Metabolites
Nutmeg
Secondary metabolites
Seeds
Sun
Ultraviolet radiation
Visual observation
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Summary:Traditional practice of nutmeg sun-drying causes some fungi such as Aspergillus flavus to grow. One of the secondary metabolites of A. flavus named aflatoxin (AFs) is known to be carcinogenic, so the dried nutmeg kernel must be aflatoxin-free in the trading. Aflatoxin detection requires time and costly, make it difficult to conduct at the farmers level. This study aims to develop a simple and low-cost method to detect aflatoxin at the farmer level. Fresh nutmeg seeds were dried in two ways; sundried everyday (continuous), and sundried every two days (intermittent), both for around 18 days. The dried nutmeg seeds are then stored in a rice sack under normal conditions until the fungi grow, then they were opened and the images of kernels were captured using a CCD camera, with normal light and UV light sources. Visual observation on images captured in normal light source was able to detect the presence of fungi on dried kernels, by 28.0% for continuous and 26.2% for intermittent sun-drying. Visual observation on images captured in UV light source was able to detect the presence of aflatoxin on dried kernels, indicated by blue luminance on kernel, by 10.4% and 13.4% for continuous and intermittent sun-drying.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/147/1/012003