Identification and abundance of nitrifying-denitrifying bacteria in malang sand filter based culture environment for mud crabs Scylla serrata

• Published in: IOP conference series. Earth and environmental science 2020-01, Vol.404 (1), p.12032
• Main Authors: Hastuti, Y P, Andina, Y, Supriyono, E, Fatma, Y S, Tridesianti, S
• Format: Article
• Language: English
• Subjects: Abundance; Bacteria; Biota; Crustaceans; Cultivation; denitrification; Denitrifying bacteria; filtration; malang sand; Metabolic wastes; Morphology; Mud; nitrification; Organic matter; Panopeidae; Sand; Sand filters; Satiety; Scylla; Scylla serrata; Survival; Water purification; Water quality; Weight
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/404/1/012032

Accumulation of organic matter derived from an uneaten feed, faeces, and other metabolic waste of biota can reduce water quality. This issue can be overcome by physical method using filtration systems such as malang sand. The present study aimed to identify the morphology and abundance of nitrifying and denitrifying bacteria in the mud crab Scylla serrata cultivation system treated with different weight of malang sand as i.e. 0 kg (control), 5 kg, 10 kg, 15 kg, and 20 kg. Mud crabs with the weight of 73.72±1.05 g and carapace length of 7.28±0.06 cm were reared in a container measuring 60 cm x 30 cm x 30 cm for 30 days fed two times a day using ad satiation method (as satisfied as possible). The results showed that a treatment using 5 kg malang sand was the best treatment with the survival rate of mud crabs reached 77.77±19.2%. At the end of cultivation period, nitrifying and denitrifying bacteria accounted for 1.52x107 CFU mL−1 and 1.06x107 CFU mL−1, respectively. The nitrifying and denitrifying bacteria in the rearing water were classified as Pseudomonas sp. and Acinetobacter sp., respectively.