The use of Superstim modifications in low and ultra-low doses for long term deposition of micro-plants of perpetual raspberry in the culture room conditions

• Published in: IOP conference series. Earth and environmental science 2021-02, Vol.663 (1), p.12003
• Main Authors: Akimova, S V, Kirkach, V V, Demenko, V I, Malevannaya, N N
• Format: Article
• Language: English
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/663/1/012003

A method for long-term deposition of micro-plants in the culture room conditions when adding Superstim-1 and Superstim-2 products in low doses to the nutrient medium has been developed for clonal micro-propagation of perpetual raspberry (Diamond, Gerakl varieties). This method does not require any special equipment and ensures viability sustaining of micro-plants without signs of necrosis and chlorosis for 10-12 months without additional transfers to a fresh nutrient medium. When depositing regenerants of the Brilliantovaya variety for 12 months in the culture room conditions, the advantage of adding Superstim-1 in concentration of 1×10-9 %, 1×10-15 %, 1×10-18 % to the nutrient medium composition was revealed. When depositing the Gerakl variety, it was found that storing of micro-plants of the Gerakl variety for more than 10 months is impractical, since at the 12-th month of sub-culturing the viability of micro-plants did not exceed 30% even in the best variants of the experiment. At the 10th month of sub-culturing, the advantage of adding the Superstim-1 product 1 1×10-6 %, 1×10-9%, 1×10-15 %, 1×10-18 % to the nutrient medium composition of the Superstim-1 product was revealed.