The creation of a Taraxacum kok-saghyz high frequency regeneration system

The aim of this research was to study the morphogenetic ability of Taraxacum kok-saghyz root tissues and to optimize the culture medium for the subsequent genetic transformation of plants. The effects of exogenous hormone exposure on survival, in vitro shoot induction, and root formation were studie...

Full description

Saved in:
Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2021-11, Vol.843 (1), p.12041
Main Authors: Verbitskaya, A A, Ivanova, A S, Konkova, N G, Gaponenko, A K
Format: Article
Language:English
Subjects:
Auxins
Culture media
Cytokinins
Explants
Genetic transformation
Growth regulators
Plant tissues
Regeneration
Rooting
Sucrose
Survival
Taraxacum
Transplantation
Vitamins
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this research was to study the morphogenetic ability of Taraxacum kok-saghyz root tissues and to optimize the culture medium for the subsequent genetic transformation of plants. The effects of exogenous hormone exposure on survival, in vitro shoot induction, and root formation were studied by using root tissues. For the cultivation the samples of kok-saghyz, the Murashige-Skuga nutrient medium was used as the basis, supplemented with sucrose 20 g/l, vitamins B5 1 mg/l, and also containing agar 5.5 g/l. The pH value is 5.7. For the plant regeneration induction, growth regulators, auxins and cytokinins were added in culture media. In this study, the roots of D 1 mm and two media variants were used for comparison: variant 1. MS + 6-BAP 1 mg/l; variant 2. MS + 6-BAP 1 mg/l + IAA 0.2 mg/l. An effective protocol for the regeneration of kok-saghyz explants was developed. There was a high percentage of regeneration of 87.6% on the medium containing a combination of cytokinin and auxin, as well as a high percentage of direct shoot formation, which was 65.1%, the degree of rooting was 100%, the resulting cultured plant tissues grew well and had a high survival rate after transplantation.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/843/1/012041