Bioanalytical Technologies for Safety Control of Fish and Seafood by Sensitive Rapid Tests for Phycotoxins

Wide and operate safety monitoring of foodstuffs is highly demanded in modern society. This work aims to develop and characterize new test systems for control of phycotoxins, dangerous contaminants of fish and seafood. For rapid and productive testing, 2 methods have been implemented: immunochromato...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2022-02, Vol.988 (4), p.42041
Main Authors: Eremin, S A, Zherdev, A V, Tokeshi, M, Hibara, A
Format: Article
Language:English
Subjects:
Antibodies
Aptamers
Contaminants
Fish
Fluorescence
Fluorescence in situ hybridization
Fluorescence polarization
Immunoassay
Phycotoxins
Pollution monitoring
Safety
Sample preparation
Seafood
Sensitivity analysis
Target detection
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Summary:Wide and operate safety monitoring of foodstuffs is highly demanded in modern society. This work aims to develop and characterize new test systems for control of phycotoxins, dangerous contaminants of fish and seafood. For rapid and productive testing, 2 methods have been implemented: immunochromatographic assay and fluorescence polarization immunoassay. Various approaches for reducing the limit of detection of target analytes have been considered, including changes in the use of optical and fluorescent labels and varied order of the detected complexes formation. Antibodies and aptamers have been considered as receptor molecules. The developed techniques provide rapid (20–30 minutes, including sample preparation) and sensitive testing. Their effectiveness has been shown for different kinds of fish and seafood. Portable detectors have been proposed that allow testing directly at the sampling points, without transportation to centralized laboratories.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/988/4/042041