Rapid and Convenient Separation of Chitooligosaccharides by Ion-Exchange Chromatography

Pervious methods for separation of highly purified chitooligosaccharides was time-consuming and labor-intensive, which limited the large-scale production. This study developed a convenient ion-exchange chromatography using the ÄKTA™ avant 150 chromatographic system. Five fractions were automatically...

Full description

Saved in:
Bibliographic Details
Published in:IOP conference series. Materials Science and Engineering 2017-12, Vol.275 (1), p.12008
Main Authors: Wu, Yuxiao, Lu, Wei-Peng, Wang, Jianing, Gao, Yunhua, Guo, Yanchuan
Format: Article
Language:English
Subjects:
Chromatography
High performance liquid chromatography
Ion exchange
Mass production
Separation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pervious methods for separation of highly purified chitooligosaccharides was time-consuming and labor-intensive, which limited the large-scale production. This study developed a convenient ion-exchange chromatography using the ÄKTA™ avant 150 chromatographic system. Five fractions were automatically collected under detecting the absorption at 210 nm. The fractions were analyzed by high-performance liquid chromatography. It proved that they primarily comprised chitobiose, chitotriose, chitotetraose, chitopentaose, and chitohexaose, respectively, with chromatographic purities over 90%. The separation process was rapid, convenient and could be monitored on-line, which would be benefit for the mass production of chitooligosaccharides.
ISSN:1757-8981
1757-899X
DOI:10.1088/1757-899X/275/1/012008