Electrochemical Sensing Methodology for Antibiogram Assays

We report an electrochemical method of determining antibiotic susceptibility by detecting the electrochemical oxidation peak signature of the hydrolyzed fluorescein ester. Conventionally, antibiotic susceptibility studies are conducted using optical techniques. However, an electrochemical approach u...

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Bibliographic Details
Published in:Journal of the Electrochemical Society 2014-01, Vol.161 (2), p.B3061-B3063
Main Authors: Menon, Shruti, Vishnu, Nandimalla, Panchapakesan, Shanker Shyam Sundhar, Kumar, Annamalai Senthil, Sankaran, Krishnan, Unrau, Peter, Parameswaran, M. Ash
Format: Article
Language:English
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Summary:We report an electrochemical method of determining antibiotic susceptibility by detecting the electrochemical oxidation peak signature of the hydrolyzed fluorescein ester. Conventionally, antibiotic susceptibility studies are conducted using optical techniques. However, an electrochemical approach used in conjunction with the popular antibiogram assay offers an avenue to move away from these expensive and time consuming colorimetric techniques. Tests were conducted using a conventional three-electrode system in samples of E.coli incubated with fluorescein diacetate, with and without the antibiotic Penicillin and Streptomycin. A peak/no-peak signature observed in a voltammetric scan indicated the presence/absence of fluorescein in the sample, suggesting that the antibiotic may/may not be considered effective. In the future, this electrochemical approach could be miniaturized onto portable electronic systems such as point-of-care antibiogram apparatus for rapid antibiotic tests.
ISSN:0013-4651
1945-7111
DOI:10.1149/2.011402jes