SAM-Based Immunosensor for the Analysis of Thyroxine (T4)

In this work T4, also known as thyroxine, a thyroid hormone with many relevant roles in human metabolism, was determined with an antibody-based biosensor, consisting on a multi thiol self-assembled monolayer (SAM) whereas a selective antibody was attached. Electroanalytical measurements were perform...

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Bibliographic Details
Published in:Journal of the Electrochemical Society 2017-01, Vol.164 (4), p.B103-B106
Main Authors: Couto, Rosa A. S., Gonçalves, Luís Moreira, Góes, Marcio Sousa, Rodrigues, Cecília M. P., Quinaz, M. Beatriz, Rodrigues, José A.
Format: Article
Language:English
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Summary:In this work T4, also known as thyroxine, a thyroid hormone with many relevant roles in human metabolism, was determined with an antibody-based biosensor, consisting on a multi thiol self-assembled monolayer (SAM) whereas a selective antibody was attached. Electroanalytical measurements were performed by impedance on a ferricyanide redox probe. A limit of detection of 1.5 ng mL−1 and limit of quantification (LOQ) of 4.0 ng mL−1 were obtained. This biosensor is a proof-of-concept for the creation of point-of-care electrochemical approach for the quantification of T4.
ISSN:0013-4651
1945-7111
DOI:10.1149/2.0561704jes