Electrochemical Invertase Probes with Nanocomposite of Microfibrillated Cellulose-Tragacanth Gum-Metal Nanoparticles for Direct Sucrose Analysis in Sweetened Beverages

• Published in: Journal of the Electrochemical Society 2019, Vol.166 (8), p.B720-B727
• Main Authors: Bagal-Kestwal, Dipali R., Chiang, Been-Huang
• Format: Article
• Language: English
• ISSN: 0013-4651; 1945-7111
• DOI: 10.1149/2.1091908jes

False claim/labels on commercial products with high-sugar can lead to serious health issues; therefore, fast analytical method for qualitative and quantitative detection of sucrose/table sugar is very important. Rapid and sensitive electrochemical sensors based on composite hydrogel matrix of microfibrillated cellulose (MFC), Indian gum Tragacanth (IGT) with reinforced nanomaterials and co-entrapped enzymes were prepared for sucrose analysis in tea samples. Gold screen printed electrodes (AuSPEs) were modified with multilayered nanocomposite of MFC-IGT as continuous matrix with invertase (INV)-glucose oxidase (GOx) and gold/copper nanoparticles (AuNPs/CuNPs) as filler and their electrochemistry was studied. Probe A (INVWM-GOx-AuNPs-MFC-IGT/AuSPE) demonstrated the sucrose detection range from 10 nM to 10 μM while Probe B (INVWM-GOx-CuNPs-MFC-IGT/AuSPE) and Probe C (INVWM-GOx- Au/CuNPs- MFC-IGT/AuSPE) displayed a wider linear range (0.1 nM to 10 μM) and better limit of detection. Based on performance, probes B and C were used to detect sucrose content in sugar-sweetened beverages i.e. tea samples. The novel nanocomposite polymeric-backbone of MFC-IGT proved its highest sensitivity towards sucrose. All modified-gold probes exhibited higher reproducibility as well as better long-term stability and reusability with fast response time (