Electrochemical Invertase Probes with Nanocomposite of Microfibrillated Cellulose-Tragacanth Gum-Metal Nanoparticles for Direct Sucrose Analysis in Sweetened Beverages

False claim/labels on commercial products with high-sugar can lead to serious health issues; therefore, fast analytical method for qualitative and quantitative detection of sucrose/table sugar is very important. Rapid and sensitive electrochemical sensors based on composite hydrogel matrix of microf...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the Electrochemical Society 2019, Vol.166 (8), p.B720-B727
Main Authors: Bagal-Kestwal, Dipali R., Chiang, Been-Huang
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c268t-6899639e32a5c3548da173535b4ee15628d0affd7824c9a3a104c42553305313
cites cdi_FETCH-LOGICAL-c268t-6899639e32a5c3548da173535b4ee15628d0affd7824c9a3a104c42553305313
container_end_page B727
container_issue 8
container_start_page B720
container_title Journal of the Electrochemical Society
container_volume 166
creator Bagal-Kestwal, Dipali R.
Chiang, Been-Huang
description False claim/labels on commercial products with high-sugar can lead to serious health issues; therefore, fast analytical method for qualitative and quantitative detection of sucrose/table sugar is very important. Rapid and sensitive electrochemical sensors based on composite hydrogel matrix of microfibrillated cellulose (MFC), Indian gum Tragacanth (IGT) with reinforced nanomaterials and co-entrapped enzymes were prepared for sucrose analysis in tea samples. Gold screen printed electrodes (AuSPEs) were modified with multilayered nanocomposite of MFC-IGT as continuous matrix with invertase (INV)-glucose oxidase (GOx) and gold/copper nanoparticles (AuNPs/CuNPs) as filler and their electrochemistry was studied. Probe A (INVWM-GOx-AuNPs-MFC-IGT/AuSPE) demonstrated the sucrose detection range from 10 nM to 10 μM while Probe B (INVWM-GOx-CuNPs-MFC-IGT/AuSPE) and Probe C (INVWM-GOx- Au/CuNPs- MFC-IGT/AuSPE) displayed a wider linear range (0.1 nM to 10 μM) and better limit of detection. Based on performance, probes B and C were used to detect sucrose content in sugar-sweetened beverages i.e. tea samples. The novel nanocomposite polymeric-backbone of MFC-IGT proved its highest sensitivity towards sucrose. All modified-gold probes exhibited higher reproducibility as well as better long-term stability and reusability with fast response time (
doi_str_mv 10.1149/2.1091908jes
format article
fullrecord <record><control><sourceid>iop_cross</sourceid><recordid>TN_cdi_iop_journals_10_1149_2_1091908jes</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1091908JES</sourcerecordid><originalsourceid>FETCH-LOGICAL-c268t-6899639e32a5c3548da173535b4ee15628d0affd7824c9a3a104c42553305313</originalsourceid><addsrcrecordid>eNptkMtOwzAQRS0EEuWx4wO8ZEHAE9tpsizlVYmXRPfR1J20rty4sl0QX8Rv4gokNqxGI905985l7AzEJYBqrspLEA00ol5R3GMDaJQuhgCwzwZCgCxUpeGQHcW4yivUajhgX7eOTAreLGltDTo-6d8pJIzEX4OfUeQfNi35M_be-PXGR5uI-44_WRN8Z2fBOoeJ5nxMzm2dj1RMAy7QYJ_P7rfr4olSxu4AGwzJGpeZnQ_8xobszN-2GZTdRj26z2gjtz1_-yBK1GfqNeU0uKB4wg46dJFOf-cxm97dTscPxePL_WQ8eixMWdWpqOqmqWRDskRtpFb1HGEotdQzRQS6Kuu5wK6bD-tSmQYlglBGlVpLKbQEecwufrC7UDFQ126CXWP4bEG0u47bsv3rOMvPf-TWb9qV34b8RPxf-g14xX--</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Electrochemical Invertase Probes with Nanocomposite of Microfibrillated Cellulose-Tragacanth Gum-Metal Nanoparticles for Direct Sucrose Analysis in Sweetened Beverages</title><source>Institute of Physics</source><creator>Bagal-Kestwal, Dipali R. ; Chiang, Been-Huang</creator><creatorcontrib>Bagal-Kestwal, Dipali R. ; Chiang, Been-Huang</creatorcontrib><description>False claim/labels on commercial products with high-sugar can lead to serious health issues; therefore, fast analytical method for qualitative and quantitative detection of sucrose/table sugar is very important. Rapid and sensitive electrochemical sensors based on composite hydrogel matrix of microfibrillated cellulose (MFC), Indian gum Tragacanth (IGT) with reinforced nanomaterials and co-entrapped enzymes were prepared for sucrose analysis in tea samples. Gold screen printed electrodes (AuSPEs) were modified with multilayered nanocomposite of MFC-IGT as continuous matrix with invertase (INV)-glucose oxidase (GOx) and gold/copper nanoparticles (AuNPs/CuNPs) as filler and their electrochemistry was studied. Probe A (INVWM-GOx-AuNPs-MFC-IGT/AuSPE) demonstrated the sucrose detection range from 10 nM to 10 μM while Probe B (INVWM-GOx-CuNPs-MFC-IGT/AuSPE) and Probe C (INVWM-GOx- Au/CuNPs- MFC-IGT/AuSPE) displayed a wider linear range (0.1 nM to 10 μM) and better limit of detection. Based on performance, probes B and C were used to detect sucrose content in sugar-sweetened beverages i.e. tea samples. The novel nanocomposite polymeric-backbone of MFC-IGT proved its highest sensitivity towards sucrose. All modified-gold probes exhibited higher reproducibility as well as better long-term stability and reusability with fast response time (&lt;5 s) as compared to previous reports. Thus, the proposed methodology can be promising tool in food and biomedical industry with slight modifications.</description><identifier>ISSN: 0013-4651</identifier><identifier>EISSN: 1945-7111</identifier><identifier>DOI: 10.1149/2.1091908jes</identifier><language>eng</language><publisher>The Electrochemical Society</publisher><ispartof>Journal of the Electrochemical Society, 2019, Vol.166 (8), p.B720-B727</ispartof><rights>2019 The Electrochemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c268t-6899639e32a5c3548da173535b4ee15628d0affd7824c9a3a104c42553305313</citedby><cites>FETCH-LOGICAL-c268t-6899639e32a5c3548da173535b4ee15628d0affd7824c9a3a104c42553305313</cites><orcidid>0000-0003-3766-4161 ; 0000-0002-0573-5650</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27902,27903,27904</link.rule.ids></links><search><creatorcontrib>Bagal-Kestwal, Dipali R.</creatorcontrib><creatorcontrib>Chiang, Been-Huang</creatorcontrib><title>Electrochemical Invertase Probes with Nanocomposite of Microfibrillated Cellulose-Tragacanth Gum-Metal Nanoparticles for Direct Sucrose Analysis in Sweetened Beverages</title><title>Journal of the Electrochemical Society</title><addtitle>J. Electrochem. Soc</addtitle><description>False claim/labels on commercial products with high-sugar can lead to serious health issues; therefore, fast analytical method for qualitative and quantitative detection of sucrose/table sugar is very important. Rapid and sensitive electrochemical sensors based on composite hydrogel matrix of microfibrillated cellulose (MFC), Indian gum Tragacanth (IGT) with reinforced nanomaterials and co-entrapped enzymes were prepared for sucrose analysis in tea samples. Gold screen printed electrodes (AuSPEs) were modified with multilayered nanocomposite of MFC-IGT as continuous matrix with invertase (INV)-glucose oxidase (GOx) and gold/copper nanoparticles (AuNPs/CuNPs) as filler and their electrochemistry was studied. Probe A (INVWM-GOx-AuNPs-MFC-IGT/AuSPE) demonstrated the sucrose detection range from 10 nM to 10 μM while Probe B (INVWM-GOx-CuNPs-MFC-IGT/AuSPE) and Probe C (INVWM-GOx- Au/CuNPs- MFC-IGT/AuSPE) displayed a wider linear range (0.1 nM to 10 μM) and better limit of detection. Based on performance, probes B and C were used to detect sucrose content in sugar-sweetened beverages i.e. tea samples. The novel nanocomposite polymeric-backbone of MFC-IGT proved its highest sensitivity towards sucrose. All modified-gold probes exhibited higher reproducibility as well as better long-term stability and reusability with fast response time (&lt;5 s) as compared to previous reports. Thus, the proposed methodology can be promising tool in food and biomedical industry with slight modifications.</description><issn>0013-4651</issn><issn>1945-7111</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNptkMtOwzAQRS0EEuWx4wO8ZEHAE9tpsizlVYmXRPfR1J20rty4sl0QX8Rv4gokNqxGI905985l7AzEJYBqrspLEA00ol5R3GMDaJQuhgCwzwZCgCxUpeGQHcW4yivUajhgX7eOTAreLGltDTo-6d8pJIzEX4OfUeQfNi35M_be-PXGR5uI-44_WRN8Z2fBOoeJ5nxMzm2dj1RMAy7QYJ_P7rfr4olSxu4AGwzJGpeZnQ_8xobszN-2GZTdRj26z2gjtz1_-yBK1GfqNeU0uKB4wg46dJFOf-cxm97dTscPxePL_WQ8eixMWdWpqOqmqWRDskRtpFb1HGEotdQzRQS6Kuu5wK6bD-tSmQYlglBGlVpLKbQEecwufrC7UDFQ126CXWP4bEG0u47bsv3rOMvPf-TWb9qV34b8RPxf-g14xX--</recordid><startdate>2019</startdate><enddate>2019</enddate><creator>Bagal-Kestwal, Dipali R.</creator><creator>Chiang, Been-Huang</creator><general>The Electrochemical Society</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0003-3766-4161</orcidid><orcidid>https://orcid.org/0000-0002-0573-5650</orcidid></search><sort><creationdate>2019</creationdate><title>Electrochemical Invertase Probes with Nanocomposite of Microfibrillated Cellulose-Tragacanth Gum-Metal Nanoparticles for Direct Sucrose Analysis in Sweetened Beverages</title><author>Bagal-Kestwal, Dipali R. ; Chiang, Been-Huang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c268t-6899639e32a5c3548da173535b4ee15628d0affd7824c9a3a104c42553305313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bagal-Kestwal, Dipali R.</creatorcontrib><creatorcontrib>Chiang, Been-Huang</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of the Electrochemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bagal-Kestwal, Dipali R.</au><au>Chiang, Been-Huang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical Invertase Probes with Nanocomposite of Microfibrillated Cellulose-Tragacanth Gum-Metal Nanoparticles for Direct Sucrose Analysis in Sweetened Beverages</atitle><jtitle>Journal of the Electrochemical Society</jtitle><addtitle>J. Electrochem. Soc</addtitle><date>2019</date><risdate>2019</risdate><volume>166</volume><issue>8</issue><spage>B720</spage><epage>B727</epage><pages>B720-B727</pages><issn>0013-4651</issn><eissn>1945-7111</eissn><abstract>False claim/labels on commercial products with high-sugar can lead to serious health issues; therefore, fast analytical method for qualitative and quantitative detection of sucrose/table sugar is very important. Rapid and sensitive electrochemical sensors based on composite hydrogel matrix of microfibrillated cellulose (MFC), Indian gum Tragacanth (IGT) with reinforced nanomaterials and co-entrapped enzymes were prepared for sucrose analysis in tea samples. Gold screen printed electrodes (AuSPEs) were modified with multilayered nanocomposite of MFC-IGT as continuous matrix with invertase (INV)-glucose oxidase (GOx) and gold/copper nanoparticles (AuNPs/CuNPs) as filler and their electrochemistry was studied. Probe A (INVWM-GOx-AuNPs-MFC-IGT/AuSPE) demonstrated the sucrose detection range from 10 nM to 10 μM while Probe B (INVWM-GOx-CuNPs-MFC-IGT/AuSPE) and Probe C (INVWM-GOx- Au/CuNPs- MFC-IGT/AuSPE) displayed a wider linear range (0.1 nM to 10 μM) and better limit of detection. Based on performance, probes B and C were used to detect sucrose content in sugar-sweetened beverages i.e. tea samples. The novel nanocomposite polymeric-backbone of MFC-IGT proved its highest sensitivity towards sucrose. All modified-gold probes exhibited higher reproducibility as well as better long-term stability and reusability with fast response time (&lt;5 s) as compared to previous reports. Thus, the proposed methodology can be promising tool in food and biomedical industry with slight modifications.</abstract><pub>The Electrochemical Society</pub><doi>10.1149/2.1091908jes</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-3766-4161</orcidid><orcidid>https://orcid.org/0000-0002-0573-5650</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0013-4651
ispartof Journal of the Electrochemical Society, 2019, Vol.166 (8), p.B720-B727
issn 0013-4651
1945-7111
language eng
recordid cdi_iop_journals_10_1149_2_1091908jes
source Institute of Physics
title Electrochemical Invertase Probes with Nanocomposite of Microfibrillated Cellulose-Tragacanth Gum-Metal Nanoparticles for Direct Sucrose Analysis in Sweetened Beverages
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T10%3A55%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-iop_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Electrochemical%20Invertase%20Probes%20with%20Nanocomposite%20of%20Microfibrillated%20Cellulose-Tragacanth%20Gum-Metal%20Nanoparticles%20for%20Direct%20Sucrose%20Analysis%20in%20Sweetened%20Beverages&rft.jtitle=Journal%20of%20the%20Electrochemical%20Society&rft.au=Bagal-Kestwal,%20Dipali%20R.&rft.date=2019&rft.volume=166&rft.issue=8&rft.spage=B720&rft.epage=B727&rft.pages=B720-B727&rft.issn=0013-4651&rft.eissn=1945-7111&rft_id=info:doi/10.1149/2.1091908jes&rft_dat=%3Ciop_cross%3E1091908JES%3C/iop_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c268t-6899639e32a5c3548da173535b4ee15628d0affd7824c9a3a104c42553305313%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true