Cell depth imaging by point laser scanning fluorescence microscopy with an optical disk pickup head

A compact, cost-effective, and position-addressable digital laser scanning microscopy (DLSM) instrument is made using a commercially available Blu-ray disc read-only memory (BD-ROM) pickup head. Fluorescent cell images captured by DLSM have resolutions of 0.38 µm. Because of the position-addressable...

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Bibliographic Details
Published in:Japanese Journal of Applied Physics 2015-09, Vol.54 (9S), p.9
Main Authors: Tsai, Rung-Ywan, Chen, Jung-Po, Lee, Yuan-Chin, Chiang, Hung-Chih, Cheng, Chih-Ming, Huang, Chun-Chieh, Huang, Tai-Ting, Cheng, Chung-Ta, Tiao, Golden
Format: Article
Language:English
Subjects:
Beams (radiation)
Excitation
Fluorescence
Laser beams
Lasers
Lenses
Optical discs
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Summary:A compact, cost-effective, and position-addressable digital laser scanning microscopy (DLSM) instrument is made using a commercially available Blu-ray disc read-only memory (BD-ROM) pickup head. Fluorescent cell images captured by DLSM have resolutions of 0.38 µm. Because of the position-addressable function, multispectral fluorescence cell images are captured using the same sample slide with different excitation laser sources. Specially designed objective lenses with the same working distance as the image-capturing beam are used for the different excitation laser sources. By accurately controlling the tilting angles of the sample slide or by moving the collimator lens of the image-capturing beam, the fluorescence cell images along different depth positions of the sample are obtained. Thus, z-section images with micrometer-depth resolutions are achievable.
ISSN:0021-4922
1347-4065
DOI:10.7567/JJAP.54.09MD01