Photoinduced enhancement of the tryptophan fluorescence of the enzyme in the luciferase — luciferin complex

An experiment providing evidence in support of the hypothesis that the bioluminescent reaction is dissociative in character was proposed and carried out. The processes occurring in the native bioluminescent system were modelled by the luciferase - luciferin complex. The experiment was performed in a...

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Bibliographic Details
Published in:Quantum electronics (Woodbury, N.Y.) N.Y.), 1999-07, Vol.29 (7), p.591-595
Main Authors: Brovko, L Yu, Dement'eva, E I, Koroteev, Nikolai I, Ugarova, N N, Cherednikova, E Yu, Chikishev, A Yu
Format: Article
Language:English
Subjects:
ALBUMINS
AMINO ACIDS
AROMATICS
AZAARENES
AZOLES
CARBOXYLIC ACIDS
CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY
CONFORMATIONAL CHANGES
DISSOCIATION
ELECTROMAGNETIC RADIATION
EMISSION
ENZYMES
FLUORESCENCE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYPOTHESIS
INDOLES
LASER RADIATION
LUCIFERASE
LUCIFERIN
LUMINESCENCE
MOLECULES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDASES
OXIDOREDUCTASES
PHOTON EMISSION
PROTEINS
PULSES
PYRROLES
QUENCHING
RADIATIONS
STEADY-STATE CONDITIONS
TRYPTOPHAN
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Summary:An experiment providing evidence in support of the hypothesis that the bioluminescent reaction is dissociative in character was proposed and carried out. The processes occurring in the native bioluminescent system were modelled by the luciferase - luciferin complex. The experiment was performed in accordance with the pump-and-probe scheme involving probing by picosecond laser pulses and was based on the steady-state quenching of the intrinsic tryptophan fluorescence of luciferase when the latter is bound in a complex with luciferin. An increase in the intensity of the tryptophan fluorescence on photoexcitation of luciferin was regarded as confirmation of the hypothesis of the dissociation of the complex and it made it possible to estimate the rate of propagation of the corresponding conformational changes through the protein molecule. (this issue is dedicated to the memory of s a akhmanov)
ISSN:1063-7818
1468-4799
DOI:10.1070/QE1999v029n07ABEH001535