Maturation of osteoblast-like SaoS2 induced by carbon nanotubes

Osteogenic maturation of the osteoblast is crucial for bone formation. In this study, multi-walled carbon nanotubes (MWCNTs) and graphite (GP) were pressed as compacts. The greater ability of carbon nanotubes to adsorb proteins, compared with graphite, was shown. Human osteoblast-like SaoS2 cells we...

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Published in:Biomedical materials (Bristol) 2009-02, Vol.4 (1), p.015005-015005
Main Authors: Li, Xiaoming, Gao, Hong, Uo, Motohiro, Sato, Yoshinori, Akasaka, Tsukasa, Abe, Shigeaki, Feng, Qingling, Cui, Fuzhai, Watari, Fumio
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Cell Differentiation
Cell Line
Cell Proliferation - drug effects
Humans
Materials Testing
Nanotubes, Carbon - chemistry
Osteoblasts - chemistry
Osteoblasts - cytology
Osteoblasts - physiology
Osteogenesis - drug effects
Osteogenesis - physiology
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Summary:Osteogenic maturation of the osteoblast is crucial for bone formation. In this study, multi-walled carbon nanotubes (MWCNTs) and graphite (GP) were pressed as compacts. The greater ability of carbon nanotubes to adsorb proteins, compared with graphite, was shown. Human osteoblast-like SaoS2 cells were cultured and the cell response to the two kinds of compacts was compared in vitro. Meanwhile, we used cell culture on the culture plate as a control. Assays for osteonectin, osteopontin and osteocalcin gene expression, total protein (TP) amount, alkaline phosphatase activity (ALP) and DNA of cells cultured on the samples were done. During the conventional culture, significantly higher osteonectin, osteopontin and osteocalcin gene expression level, ALP/DNA and TP/DNA on carbon nanotubes were found. To confirm the hypothesis that the larger amount of specific proteins adsorbed on the carbon nanotubes was crucial for this, the compacts were pre-soaked in culture medium having additional recombinant human bone morphogenetic protein-2 (rhBMP-2) before cell culture. Compared with GP, osteonectin, osteopontin and osteocalcin gene expression level, ALP/DNA and TP/DNA of the cells tested increased more on the MWCNTs after the compacts were pre-soaked in the culture medium with rhBMP-2. The results indicated that the carbon nanotubes might induce osteogenic maturation of the osteoblast by adsorbing more specific proteins.
ISSN:1748-605X
1748-6041
1748-605X
DOI:10.1088/1748-6041/4/1/015005