Flux Studies and Compartmentation Analysis of Gibberellin A1 in Oat Coleoptiles

A quantitative description using minimal assumptions has been made of the fate of GA1 upon entry into oat coleoptile parenchyma cells. Two pools of GA1 are present. Evidence from compartmentation studies is provided that these are located in the cytoplasm and the vacuole. A third pool, a metabolite...

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Bibliographic Details
Published in:Journal of experimental botany 1981-02, Vol.32 (1), p.103-119
Main Authors: DRAKE, G. A., CARR, D. J.
Format: Article
Language:English
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Summary:A quantitative description using minimal assumptions has been made of the fate of GA1 upon entry into oat coleoptile parenchyma cells. Two pools of GA1 are present. Evidence from compartmentation studies is provided that these are located in the cytoplasm and the vacuole. A third pool, a metabolite of GA1 (probably GAg) is also located within the vacuole. The concentration of GA1 within the vacuole appears to be determined by the passive equilibration of undissociated GA1 between cytoplasm and vacuole. However the cytoplasmic concentration of GA1 is also governed by the permeability of the plasma membrane to dissociated GA1. Evidence is presented which demonstrates that GA1 for metabolism is derived from the cytoplasmic pool of GA1 and that metabolism takes place within the cytoplasm. There is no evidence to indicate that the chloroplasts or etioplasts are sites for pools of GA1.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/32.1.103