α -fetoprotein and Albumin Genes are in Tandem in the Mouse Genome

The murine α fetoprotein (AFP) and serum albumin genes most probably arose in evolution as the consequence of a duplication of a common ancestral gene. They have both been previously mapped to chromosome 5 in the mouse. We now have evidence that these genes are closely linked. By using a unique copy...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1981-08, Vol.78 (8), p.4694-4698
Main Authors: Ingram, Robert S., Scott, Richard W., Tilghman, Shirley M.
Format: Article
Language:English
Subjects:
Albumins
Albumins - genetics
alpha-Fetoproteins - genetics
Animals
Bacteriophage lambda
Bacteriophages
Chromosome Mapping
Chromosomes
Cloning, Molecular
Complementary DNA
DNA
DNA probes
DNA, Recombinant
Genes
Genetic Linkage
Genetic loci
Genomics
Mice - genetics
Nucleotide sequences
Transcription, Genetic
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The murine α fetoprotein (AFP) and serum albumin genes most probably arose in evolution as the consequence of a duplication of a common ancestral gene. They have both been previously mapped to chromosome 5 in the mouse. We now have evidence that these genes are closely linked. By using a unique copy DNA probe derived from previously cloned AFP 5′flanking DNA, a recombinant DNA phage has been isolated, from a bacteriophage DNA library, that contains sequences flanking the 5′end of the AFP gene and the 3′end of the albumin gene. Restriction endonuclease mapping and DNA sequence determination of the recombinant phage and comparison to total genomic DNA confirmed that the genes are in tandem, 13.5 kilobase pairs apart, with the albumin gene to the 5′side of the AFP gene. Thus, they are transcribed from the same strand of DNA.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.78.8.4694