Identification, Characterization, and Distribution of Secretin Immunoreactivity in Rat and Pig Brain

Secretin immunoreactivity was detected in the central nervous system of the rat and pig with a highly specific radioimmunoassay. The secretin immunoreactivity in the rat and pig brain and duodenum extracts was fractionated by using a reverse-phase high-pressure liquid chromatographic system. The imm...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1981-08, Vol.78 (8), p.5221-5224
Main Authors: O'Donohue, Thomas L., Charlton, Clivel G., Miller, Russell L., Boden, Guenther, Jacobowitz, David M.
Format: Article
Language:English
Subjects:
Animals
Brain
Brain - metabolism
Brain Chemistry
Cerebral cortex
Chromatography, High Pressure Liquid
Duodenum
Hormones
Liquid chromatography
Liquids
Neurobiology
Phosphates
Radioimmunoassay
Rats
Secretin - immunology
Secretin - isolation & purification
Secretin - metabolism
Swine
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Summary:Secretin immunoreactivity was detected in the central nervous system of the rat and pig with a highly specific radioimmunoassay. The secretin immunoreactivity in the rat and pig brain and duodenum extracts was fractionated by using a reverse-phase high-pressure liquid chromatographic system. The immunoreactive secretin from pig brain and duodenum coeluted precisely with synthetic porcine secretin. However, immunoreactive secretin extracted from rat brain and duodenum eluted slightly before porcine secretin. These data suggest a slight difference in the structure of rat and pig secretin. The detection of secretin in the brain lays the groundwork for studies to determine the role of the peptide in central nervous system function.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.78.8.5221