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High Ammonia and Low pH in the Urine of the Ghost Crab, Ocypode quadrata
Nitrogen excreted into the urine (100 mM were found in the urine of laboratory-held Ocypode quadrata, suggesting that this notion might not be applicable to all crustaceans. To address this issue, hemolymph and urine were removed from freshly captured O. quadrata and analyzed for nitrogenous catabol...
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Published in: | The Biological bulletin (Lancaster) 1994-06, Vol.186 (3), p.342-348 |
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description | Nitrogen excreted into the urine (100 mM were found in the urine of laboratory-held Ocypode quadrata, suggesting that this notion might not be applicable to all crustaceans. To address this issue, hemolymph and urine were removed from freshly captured O. quadrata and analyzed for nitrogenous catabolites and major ions. Hemolymph composition was similar to that of other crustaceans, but the urine was acidic (X̄ pH = 5.50) and contained ammonia, often at >100 mM. Other nitrogenous catabolites in the urine (urea, amino acids, and uric acid) were much less concentrated: totaling |
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C. ; Wolcott, D. L. ; Holliday, C. W.</creator><creatorcontrib>De Vries, M. C. ; Wolcott, D. L. ; Holliday, C. W.</creatorcontrib><description>Nitrogen excreted into the urine (<1 mM) has generally been considered a negligible component of total nitrogen output of crustaceans. But concentrations of ammonia >100 mM were found in the urine of laboratory-held Ocypode quadrata, suggesting that this notion might not be applicable to all crustaceans. To address this issue, hemolymph and urine were removed from freshly captured O. quadrata and analyzed for nitrogenous catabolites and major ions. Hemolymph composition was similar to that of other crustaceans, but the urine was acidic (X̄ pH = 5.50) and contained ammonia, often at >100 mM. Other nitrogenous catabolites in the urine (urea, amino acids, and uric acid) were much less concentrated: totaling <12 mM on average. The ionic composition of the urine was similar to that of other crustaceans, with the exception that Na was much less concentrated than Cl-. Total osmolality of hemolymph and urine was similar. The Na+/ K+ATPase activity was greater in the antennal glands than in the posterior gills of O. quadrata, suggesting that this enzyme is important for ammonia concentration and Na resorption. This pattern of enzyme activity was not present in two terrestrial brachyurans whose urine contains little ammonia. The evolutionary significance of high ammonia concentrations in the urine of ghost crabs is unclear.</description><identifier>ISSN: 0006-3185</identifier><identifier>EISSN: 1939-8697</identifier><identifier>DOI: 10.2307/1542280</identifier><identifier>PMID: 29281344</identifier><language>eng</language><publisher>United States: Marine Biological Laboratory</publisher><subject>Adenosine triphosphatases ; Ammonia ; Analysis ; Crabs ; Crustaceans ; Digestive system ; Excretion ; Hemolymph ; Marine ; Marine biology ; Maxillary glands ; Nitrogen ; Nitrogen metabolism ; Ocypode quadrata ; Physiological aspects ; Physiology ; Urinalysis ; Urine</subject><ispartof>The Biological bulletin (Lancaster), 1994-06, Vol.186 (3), p.342-348</ispartof><rights>Copyright 1994 The Marine Biological Laboratory</rights><rights>Copyright © 1994 by Marine Biological Laboratory</rights><rights>COPYRIGHT 1994 University of Chicago Press</rights><rights>Copyright Marine Biological Laboratory Jun 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-118e1eb7e8a299b8ef41094277a2017d8d32a8141b39320a2aaa9797d88880003</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1542280$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1542280$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29281344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Vries, M. C.</creatorcontrib><creatorcontrib>Wolcott, D. L.</creatorcontrib><creatorcontrib>Holliday, C. W.</creatorcontrib><title>High Ammonia and Low pH in the Urine of the Ghost Crab, Ocypode quadrata</title><title>The Biological bulletin (Lancaster)</title><addtitle>Biol Bull</addtitle><description>Nitrogen excreted into the urine (<1 mM) has generally been considered a negligible component of total nitrogen output of crustaceans. But concentrations of ammonia >100 mM were found in the urine of laboratory-held Ocypode quadrata, suggesting that this notion might not be applicable to all crustaceans. To address this issue, hemolymph and urine were removed from freshly captured O. quadrata and analyzed for nitrogenous catabolites and major ions. Hemolymph composition was similar to that of other crustaceans, but the urine was acidic (X̄ pH = 5.50) and contained ammonia, often at >100 mM. Other nitrogenous catabolites in the urine (urea, amino acids, and uric acid) were much less concentrated: totaling <12 mM on average. The ionic composition of the urine was similar to that of other crustaceans, with the exception that Na was much less concentrated than Cl-. Total osmolality of hemolymph and urine was similar. The Na+/ K+ATPase activity was greater in the antennal glands than in the posterior gills of O. quadrata, suggesting that this enzyme is important for ammonia concentration and Na resorption. This pattern of enzyme activity was not present in two terrestrial brachyurans whose urine contains little ammonia. 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C.</au><au>Wolcott, D. L.</au><au>Holliday, C. W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High Ammonia and Low pH in the Urine of the Ghost Crab, Ocypode quadrata</atitle><jtitle>The Biological bulletin (Lancaster)</jtitle><addtitle>Biol Bull</addtitle><date>1994-06-01</date><risdate>1994</risdate><volume>186</volume><issue>3</issue><spage>342</spage><epage>348</epage><pages>342-348</pages><issn>0006-3185</issn><eissn>1939-8697</eissn><abstract>Nitrogen excreted into the urine (<1 mM) has generally been considered a negligible component of total nitrogen output of crustaceans. But concentrations of ammonia >100 mM were found in the urine of laboratory-held Ocypode quadrata, suggesting that this notion might not be applicable to all crustaceans. To address this issue, hemolymph and urine were removed from freshly captured O. quadrata and analyzed for nitrogenous catabolites and major ions. Hemolymph composition was similar to that of other crustaceans, but the urine was acidic (X̄ pH = 5.50) and contained ammonia, often at >100 mM. Other nitrogenous catabolites in the urine (urea, amino acids, and uric acid) were much less concentrated: totaling <12 mM on average. The ionic composition of the urine was similar to that of other crustaceans, with the exception that Na was much less concentrated than Cl-. Total osmolality of hemolymph and urine was similar. The Na+/ K+ATPase activity was greater in the antennal glands than in the posterior gills of O. quadrata, suggesting that this enzyme is important for ammonia concentration and Na resorption. This pattern of enzyme activity was not present in two terrestrial brachyurans whose urine contains little ammonia. The evolutionary significance of high ammonia concentrations in the urine of ghost crabs is unclear.</abstract><cop>United States</cop><pub>Marine Biological Laboratory</pub><pmid>29281344</pmid><doi>10.2307/1542280</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adenosine triphosphatases Ammonia Analysis Crabs Crustaceans Digestive system Excretion Hemolymph Marine Marine biology Maxillary glands Nitrogen Nitrogen metabolism Ocypode quadrata Physiological aspects Physiology Urinalysis Urine |
title | High Ammonia and Low pH in the Urine of the Ghost Crab, Ocypode quadrata |
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