Production and Characterization of Monoclonal Antibodies to Budgerigar Fledgling Disease Virus Major Capsid Protein VP1

Eleven hybridoma cell lines producing monoclonal antibodies (MAbs) against intact budgerigar fledgling disease (BFD) virions were produced and characterized. These antibodies were selected for their ability to react with BFD virions in an enzyme-linked immunosorbent assay. Each of these antibodies w...

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Bibliographic Details
Published in:Avian diseases 1992-07, Vol.36 (3), p.543-553
Main Authors: Fattaey, Ali, Lenz, Laurel, Consigli, Richard A.
Format: Article
Language:English
Subjects:
Antibodies
Budgerigars
Capsid proteins
Cell lines
Cellulose nitrate
Hybridomas
Polyomavirus
Reactivity
Virions
Viruses
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Summary:Eleven hybridoma cell lines producing monoclonal antibodies (MAbs) against intact budgerigar fledgling disease (BFD) virions were produced and characterized. These antibodies were selected for their ability to react with BFD virions in an enzyme-linked immunosorbent assay. Each of these antibodies was reactive in the immunofluorescent detection of BFD virus-infected cells. These antibodies immunoprecipitated intact virions and specifically recognized the major capsid protein, VP1, of the dissociated virion. The MAbs were found to preferentially recognize native BFD virus capsid protein when compared with denatured virus protein. These MAbs were capable of detecting BFD virus protein in chicken embryonated cell-culture lysates by dot-blot analysis. /// Se produjeron y caracterizaron 11 líneas celulares de hibridomas productoras de anticuerpos monoclonales contra el virus de la enfermedad del plumaje de los periquitos. Los anticuerpos monoclonales fueron seleccionados por su capacidad de reaccionar con el virus en un inmunoensayo con enzimas asociadas. Cada uno de estos anticuerpos reaccionaron en una prueba de inmunofluorescencia con células infectadas con el virus de la enfermedad del plumaje de los periquitos. Los anticuerpos monoclonales inmunoprecipitatron el virus intacto y reconocieron específicamente la proteína mayor de la cápsida VP1del virus disociado. Se encontró que los anticuerpos monoclonales reconocieron preferencialmente proteína nativa de la cápsida cuando fue comparada con la proteína viral desnaturalizada. Estos anticuerpos monoclonales fueron capaces de detectar proteína del virus de la enfermedad del plumaje de los periquitos en cultivos celulares de embriones de pollos por medio del análisis puntual.
ISSN:0005-2086
1938-4351
DOI:10.2307/1591747