Hydrolytic and transferase activities of invertases from physiological races of Phytophthora parasitica var. nicotianae [tobacco black-shank]

Race 3 of Phytophthora parasitica var. nicotianae, the tobacco blackshank fungus, utilizes sucrose less readily than races 0 and 1 of this pathogen. Races 0 and 1 produce kestoses (fructosylsucrose) from sucrose but race 3 does not. Invertases commonly possess both hydrolytic and transferase (transf...

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Bibliographic Details
Published in:Mycologia 1980-07, Vol.72 (4), p.749-758
Main Authors: Hankin, Lester, McIntyre, John L.
Format: Article
Language:English
Subjects:
Acetates
Enzymes
Homogenization
Liquids
Mycelium
Parasitism
Pathogens
Phytopathology
Quaternary ammonium compounds
Sulfates
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Summary:Race 3 of Phytophthora parasitica var. nicotianae, the tobacco blackshank fungus, utilizes sucrose less readily than races 0 and 1 of this pathogen. Races 0 and 1 produce kestoses (fructosylsucrose) from sucrose but race 3 does not. Invertases commonly possess both hydrolytic and transferase (transfructosylation) functions. Mycelia from races 0 and 1 contained more invertase (based on hydrolytic activity per g of wet mycelia) than did mycelia from race 3. However, this difference did not explain lack of transferase activity in race 3 since invertases (both crude homogenates of mycelia and ammonium sulfate fractions) of races 0 and 1 diluted to 200-fold less than that found in race 3 still exhibited the transferase function. Data show both qualitative and quantitative differences in hydrolytic and transferase activities of invertase from three physiologic races of P. parasitica var. nicotianae.
ISSN:0027-5514
1557-2536
DOI:10.1080/00275514.1980.12021243