The genetic population structure of Pyrenopeziza betulicola, the causative agent of birch leaf spot disease

Pyrenopeziza betulicola is a causative agent of leaf spot disease on birch. Within P. betulicola significant length variation (nine alleles) in 18S ribosomal genes was observed. The level of geographic differentiation among all locations was moderate but statistically significant, indicating that th...

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Bibliographic Details
Published in:Mycologia 2001-03, Vol.93 (2), p.258-264
Main Authors: Paavolainen, Laura, Kurkela, Timo, Suhonen, Johanna, Hantula, Jarkko
Format: Article
Language:English
Subjects:
18S rDNA
Alleles
Betula
DGGE
DNA
Fungi
Gels
Genetic variation
Genetics/Molecular Biology
Infections
Leaves
Microbial genetics
Polymerase chain reaction
Pyrenopeziza betulicola
RAMS
Ribosomal DNA
rRNA 18S
specific primer
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Summary:Pyrenopeziza betulicola is a causative agent of leaf spot disease on birch. Within P. betulicola significant length variation (nine alleles) in 18S ribosomal genes was observed. The level of geographic differentiation among all locations was moderate but statistically significant, indicating that the spore dispersal of P. betulicola occurs mainly on a limited scale. Within one leaf, in situ analysis of a multiallelic molecular marker revealed genetically distinct individuals in different spots, indicating that multiple colonization by P. betulicola is a common occurrence on birch leaves. Within one spot only one allele was found showing that the separate necrotic spots are usually formed by only one viable spore. Technically, the application of a specific marker combined with DGGE turned out to be a powerful method to study genetic diversity of fungi in situ.
ISSN:0027-5514
1557-2536
DOI:10.1080/00275514.2001.12063156