Use of Synthetic Oligonucleotides as Hybridization Probes: Isolation of Cloned cDNA Sequences for Human β2-microglobulin

We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human β2-microglobulin Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) an...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1981-11, Vol.78 (11), p.6613-6617
Main Authors: Suggs, Sidney V., Wallace, R. Bruce, Hirose, Tadaaki, Kawashima, Eric H., Itakura, Keiichi
Format: Article
Language:English
Subjects:
Amino acids
Biochemistry
Complementary DNA
DNA
DNA probes
Nucleotide sequences
Oligonucleotide probes
Oligonucleotides
Open reading frames
Plasmids
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Summary:We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human β2-microglobulin Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) and inserted into the plasmid vector pBR322. One β2-microglobulin cDNA clone was detected in the 535 bacterial plasmid clones that were screened. The clone has been characterized by blotting and nucleotide sequence analysis. The cloned β2-microglobulin sequence contains 217 base pairs of the 3′untranslated region of the mRNA and 328 base pairs (97%) of the coding region.
ISSN:0027-8424
DOI:10.1073/pnas.78.11.6613