Use of Synthetic Oligonucleotides as Hybridization Probes: Isolation of Cloned cDNA Sequences for Human β2-microglobulin

We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human β2-microglobulin Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) an...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1981-11, Vol.78 (11), p.6613-6617
Main Authors: Suggs, Sidney V., Wallace, R. Bruce, Hirose, Tadaaki, Kawashima, Eric H., Itakura, Keiichi
Format: Article
Language:English
Subjects:
Amino acids
Biochemistry
Complementary DNA
DNA
DNA probes
Nucleotide sequences
Oligonucleotide probes
Oligonucleotides
Open reading frames
Plasmids
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 6617
container_issue 11
container_start_page 6613
container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 78
creator Suggs, Sidney V.
Wallace, R. Bruce
Hirose, Tadaaki
Kawashima, Eric H.
Itakura, Keiichi
description We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human β2-microglobulin Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) and inserted into the plasmid vector pBR322. One β2-microglobulin cDNA clone was detected in the 535 bacterial plasmid clones that were screened. The clone has been characterized by blotting and nucleotide sequence analysis. The cloned β2-microglobulin sequence contains 217 base pairs of the 3′untranslated region of the mRNA and 328 base pairs (97%) of the coding region.
doi_str_mv 10.1073/pnas.78.11.6613
format article
fullrecord <record><control><sourceid>jstor</sourceid><recordid>TN_cdi_jstor_primary_11313</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>11313</jstor_id><sourcerecordid>11313</sourcerecordid><originalsourceid>FETCH-LOGICAL-j87t-aa4b0821f8ae2e0ba7737f4a81f123b0ccfc8ea9317dd9c72887e953d018e9563</originalsourceid><addsrcrecordid>eNotjE9LwzAcQHNQcE7Pgqd8gdb8kq5JvY36Z4PhhM3zSNNkZqTJTNpD_Vh-ED-Tg3l68OA9hO6A5EA4ezh6mXIucoC8LIFdoAkhlGeioMUVuk7pQAipZoJM0PiRNA4Gb0bff-reKrx2dh_8oJwOvW11wjLhxdhE29pv2dvg8XsMjU6PeJmCO5vToHbB6xarp7c53uivQXt1ak2IeDF00uPfH5p1VsWwd6EZnPU36NJIl_TtP6do-_K8rRfZav26rOer7CB4n0lZNERQMEJqqkkjOWfcFFKAAcoaopRRQsuKAW_bSnEqBNfVjLUExIklm6L78_aQ-hB3x2g7GccdAAPG_gDkj1x7</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Use of Synthetic Oligonucleotides as Hybridization Probes: Isolation of Cloned cDNA Sequences for Human β2-microglobulin</title><source>NCBI_PubMed Central（免费）</source><source>JSTOR Archival Journals</source><creator>Suggs, Sidney V. ; Wallace, R. Bruce ; Hirose, Tadaaki ; Kawashima, Eric H. ; Itakura, Keiichi</creator><creatorcontrib>Suggs, Sidney V. ; Wallace, R. Bruce ; Hirose, Tadaaki ; Kawashima, Eric H. ; Itakura, Keiichi</creatorcontrib><description>We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human β2-microglobulin Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) and inserted into the plasmid vector pBR322. One β2-microglobulin cDNA clone was detected in the 535 bacterial plasmid clones that were screened. The clone has been characterized by blotting and nucleotide sequence analysis. The cloned β2-microglobulin sequence contains 217 base pairs of the 3′untranslated region of the mRNA and 328 base pairs (97%) of the coding region.</description><identifier>ISSN: 0027-8424</identifier><identifier>DOI: 10.1073/pnas.78.11.6613</identifier><language>eng</language><publisher>National Academy of Sciences of the United States of America</publisher><subject>Amino acids ; Biochemistry ; Complementary DNA ; DNA ; DNA probes ; Nucleotide sequences ; Oligonucleotide probes ; Oligonucleotides ; Open reading frames ; Plasmids</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1981-11, Vol.78 (11), p.6613-6617</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/11313$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/11313$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,58236,58469</link.rule.ids></links><search><creatorcontrib>Suggs, Sidney V.</creatorcontrib><creatorcontrib>Wallace, R. Bruce</creatorcontrib><creatorcontrib>Hirose, Tadaaki</creatorcontrib><creatorcontrib>Kawashima, Eric H.</creatorcontrib><creatorcontrib>Itakura, Keiichi</creatorcontrib><title>Use of Synthetic Oligonucleotides as Hybridization Probes: Isolation of Cloned cDNA Sequences for Human β2-microglobulin</title><title>Proceedings of the National Academy of Sciences - PNAS</title><description>We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human β2-microglobulin Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) and inserted into the plasmid vector pBR322. One β2-microglobulin cDNA clone was detected in the 535 bacterial plasmid clones that were screened. The clone has been characterized by blotting and nucleotide sequence analysis. The cloned β2-microglobulin sequence contains 217 base pairs of the 3′untranslated region of the mRNA and 328 base pairs (97%) of the coding region.</description><subject>Amino acids</subject><subject>Biochemistry</subject><subject>Complementary DNA</subject><subject>DNA</subject><subject>DNA probes</subject><subject>Nucleotide sequences</subject><subject>Oligonucleotide probes</subject><subject>Oligonucleotides</subject><subject>Open reading frames</subject><subject>Plasmids</subject><issn>0027-8424</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNotjE9LwzAcQHNQcE7Pgqd8gdb8kq5JvY36Z4PhhM3zSNNkZqTJTNpD_Vh-ED-Tg3l68OA9hO6A5EA4ezh6mXIucoC8LIFdoAkhlGeioMUVuk7pQAipZoJM0PiRNA4Gb0bff-reKrx2dh_8oJwOvW11wjLhxdhE29pv2dvg8XsMjU6PeJmCO5vToHbB6xarp7c53uivQXt1ak2IeDF00uPfH5p1VsWwd6EZnPU36NJIl_TtP6do-_K8rRfZav26rOer7CB4n0lZNERQMEJqqkkjOWfcFFKAAcoaopRRQsuKAW_bSnEqBNfVjLUExIklm6L78_aQ-hB3x2g7GccdAAPG_gDkj1x7</recordid><startdate>19811101</startdate><enddate>19811101</enddate><creator>Suggs, Sidney V.</creator><creator>Wallace, R. Bruce</creator><creator>Hirose, Tadaaki</creator><creator>Kawashima, Eric H.</creator><creator>Itakura, Keiichi</creator><general>National Academy of Sciences of the United States of America</general><scope/></search><sort><creationdate>19811101</creationdate><title>Use of Synthetic Oligonucleotides as Hybridization Probes: Isolation of Cloned cDNA Sequences for Human β2-microglobulin</title><author>Suggs, Sidney V. ; Wallace, R. Bruce ; Hirose, Tadaaki ; Kawashima, Eric H. ; Itakura, Keiichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j87t-aa4b0821f8ae2e0ba7737f4a81f123b0ccfc8ea9317dd9c72887e953d018e9563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Amino acids</topic><topic>Biochemistry</topic><topic>Complementary DNA</topic><topic>DNA</topic><topic>DNA probes</topic><topic>Nucleotide sequences</topic><topic>Oligonucleotide probes</topic><topic>Oligonucleotides</topic><topic>Open reading frames</topic><topic>Plasmids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suggs, Sidney V.</creatorcontrib><creatorcontrib>Wallace, R. Bruce</creatorcontrib><creatorcontrib>Hirose, Tadaaki</creatorcontrib><creatorcontrib>Kawashima, Eric H.</creatorcontrib><creatorcontrib>Itakura, Keiichi</creatorcontrib><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suggs, Sidney V.</au><au>Wallace, R. Bruce</au><au>Hirose, Tadaaki</au><au>Kawashima, Eric H.</au><au>Itakura, Keiichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of Synthetic Oligonucleotides as Hybridization Probes: Isolation of Cloned cDNA Sequences for Human β2-microglobulin</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><date>1981-11-01</date><risdate>1981</risdate><volume>78</volume><issue>11</issue><spage>6613</spage><epage>6617</epage><pages>6613-6617</pages><issn>0027-8424</issn><abstract>We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human β2-microglobulin Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) and inserted into the plasmid vector pBR322. One β2-microglobulin cDNA clone was detected in the 535 bacterial plasmid clones that were screened. The clone has been characterized by blotting and nucleotide sequence analysis. The cloned β2-microglobulin sequence contains 217 base pairs of the 3′untranslated region of the mRNA and 328 base pairs (97%) of the coding region.</abstract><pub>National Academy of Sciences of the United States of America</pub><doi>10.1073/pnas.78.11.6613</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0027-8424
ispartof Proceedings of the National Academy of Sciences - PNAS, 1981-11, Vol.78 (11), p.6613-6617
issn 0027-8424
language eng
recordid cdi_jstor_primary_11313
source NCBI_PubMed Central（免费）; JSTOR Archival Journals
subjects Amino acids
Biochemistry
Complementary DNA
DNA
DNA probes
Nucleotide sequences
Oligonucleotide probes
Oligonucleotides
Open reading frames
Plasmids
title Use of Synthetic Oligonucleotides as Hybridization Probes: Isolation of Cloned cDNA Sequences for Human β2-microglobulin
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T12%3A48%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Use%20of%20Synthetic%20Oligonucleotides%20as%20Hybridization%20Probes:%20Isolation%20of%20Cloned%20cDNA%20Sequences%20for%20Human%20%CE%B22-microglobulin&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Suggs,%20Sidney%20V.&rft.date=1981-11-01&rft.volume=78&rft.issue=11&rft.spage=6613&rft.epage=6617&rft.pages=6613-6617&rft.issn=0027-8424&rft_id=info:doi/10.1073/pnas.78.11.6613&rft_dat=%3Cjstor%3E11313%3C/jstor%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-j87t-aa4b0821f8ae2e0ba7737f4a81f123b0ccfc8ea9317dd9c72887e953d018e9563%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_jstor_id=11313&rfr_iscdi=true