Growth of Purified Astrocytes in a Chemically Defined Medium

Astrocytes purified from primary cultures of neonatal rat cerebrum can now be grown in a synthetic medium supplemented with putrescine, prostaglandin F2α, insulin, fibroblast growth factor, and hydrocortisone. These five supplements have a marked synergistic effect on growth when used in combination...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1981-11, Vol.78 (11), p.7205-7209
Main Authors: Morrison, Richard S., De Vellis, Jean
Format: Article
Language:English
Subjects:
Animals
Astrocytes
Astrocytes - drug effects
Astrocytes - physiology
Brain - physiology
Cell culture techniques
Cell Division - drug effects
Cell growth
Cell lines
Cells, Cultured
Culture Media
Cultured cells
Dinoprost
Dose response relationship
Fibroblast Growth Factors
Glial Fibrillary Acidic Protein
Growth Substances - pharmacology
Hydrocortisone - pharmacology
Insulin
Insulin - pharmacology
Kinetics
Nerve Tissue Proteins - metabolism
Neuroglia
Neurons
Peptides - pharmacology
Prostaglandins
Prostaglandins F - pharmacology
Putrescine - pharmacology
Rats
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Astrocytes purified from primary cultures of neonatal rat cerebrum can now be grown in a synthetic medium supplemented with putrescine, prostaglandin F2α, insulin, fibroblast growth factor, and hydrocortisone. These five supplements have a marked synergistic effect on growth when used in combination but have little effect when used individually. Astrocytes grown in the defined medium exhibit dramatic changes in morphological characteristics in comparison to cells grown in serum-free or serum-supplemented medium. In addition, these cells express the astrocyte-specific marker glial fibrillary acidic protein and are estimated by several criteria to be greater than 95% astrocytes.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.78.11.7205