Assignment of Human α1-antitrypsin to Chromosome 14 by Somatic Cell Hybrid Analysis

• Published in: Proceedings of the National Academy of Sciences - PNAS 1982-02, Vol.79 (3), p.870-873
• Main Authors: Darlington, Gretchen J., Astrin, Kenneth H., Muirhead, Susan P., Desnick, Robert J., Smith, Moyra
• Format: Article
• Language: English
• Subjects: Cell lines; Chromosomes; Enzymes; Genes; Hepatocellular carcinoma; Hepatocytes; Human chromosomes; Hybrid cells; Hybridity; Somatic cells
• ISSN: 0027-8424

Human α1-antitrypsin (α -1-AT;Pi) production was analyzed in 11 primary mouse hepatoma-human lymphoid cell hybrids and in 14 secondary rat hepatoma-human fetal liver fibroblast hybrids. The presence of human α -1-AT was determined by Laurell immunoelectrophoresis of concentrated and isotopically labeled supernatant medium. Human α -1-AT production segregated in the mouse-human hybrids concordantly with human purine nucleoside phosphorylase and with chromosome 14. All rat-human hybrids that were α -1-AT positive were also positive for human purine nucleoside phosphorylase and chromosome 14. Our study demonstrated the usefulness of rodent hepatoma cell hybrids for mapping human liver-specific genes because differentiated functions are expressed despite the fact that the human parental cells did not express these functions. Our study also showed that human α -1-AT gene product can be processed for secretion in the rodent hepatoma cellular environment. The mouse-human hybrids showed that no other human chromosome carries genes necessary for processing or secretion of human α -1-AT in the hybrid cell milieu.