Localization of a Peripheral Membrane Protein: Gβ γ Targets GαZ

To explore the relative roles of protein-binding partners vs. lipid modifications in controlling membrane targeting of a typical peripheral membrane protein, Gαz, we directed its binding partner, β γ , to mislocalize on mitochondria. Mislocalized β γ directed wild-type Gαzand a palmitate-lacking Gαz...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2000-02, Vol.97 (3), p.1085-1090
Main Authors: Fishburn, C. Simone, Pollitt, Sonia K., Bourne, Henry R.
Format: Article
Language:English
Subjects:
Antibodies
Biological Sciences
Cell membranes
CHO cells
Fluorescence
Fluorescent antibody techniques
Lipids
Membrane proteins
Mitochondria
Palmitates
Proteins
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Summary:To explore the relative roles of protein-binding partners vs. lipid modifications in controlling membrane targeting of a typical peripheral membrane protein, Gαz, we directed its binding partner, β γ , to mislocalize on mitochondria. Mislocalized β γ directed wild-type Gαzand a palmitate-lacking Gαzmutant to mitochondria but did not alter localization of a Gαzmutant lacking both myristate and palmitate. Thus, in this paradigm, a protein-protein interaction controls targeting of a peripheral membrane protein to the proper compartment, whereas lipid modifications stabilize interactions of proteins with membranes and with other proteins.
ISSN:0027-8424