Antigen-Specific Human T-Cell Hybridomas with Helper Activity

Human T cell hybridomas were produced by fusing the hypoxanthine phosphoribosyltransferase-deficient line of the human T cell lymphoma Jurkat with a continuous line of normal human T cells specific for tetanus toxoid (TeT). The hybridomas were selected for their ability to produce interleukin 2 afte...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1982-11, Vol.79 (21), p.6646-6650
Main Authors: DeFreitas, Elaine C., Vella, Stefano, Linnenbach, Alban, Zmijewski, Chester, Koprowski, Hilary, Croce, Carlo M.
Format: Article
Language:English
Subjects:
Antibodies
Antibody Formation
Antigens
B lymphocytes
B-Lymphocytes - immunology
Cell Line
Cell lines
Cultured cells
Humans
Hybridity
Hybridomas
Hybridomas - immunology
Interleukin-2 - biosynthesis
Lymphocyte Cooperation
Monocytes
Phenotypes
Receptors, Antigen, T-Cell - immunology
T lymphocytes
T-Lymphocytes - immunology
Tetanus Toxoid - immunology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human T cell hybridomas were produced by fusing the hypoxanthine phosphoribosyltransferase-deficient line of the human T cell lymphoma Jurkat with a continuous line of normal human T cells specific for tetanus toxoid (TeT). The hybridomas were selected for their ability to produce interleukin 2 after exposure to TeT on semiautologous monocytes and for their ability to bind to TeT-pulsed semiautologous monocytes. These antigen-specific T hybridomas demonstrated potent helper activity for semiautologous B cells as determined by the production of high levels of anti-TeT antibody in vitro.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.79.21.6646