Spermidine/Spermine N1-Acetyltransferase Specifically Binds to the Integrin α9 Subunit Cytoplasmic Domain and Enhances Cell Migration

The integrin α9β1 is expressed on migrating cells, such as leukocytes, and binds to multiple ligands that are present at sites of tissue injury and inflammation. α9β1, like the structurally related integrin α4β1, mediates accelerated cell migration, an effect that depends on the α9 cytoplasmic domai...

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Bibliographic Details
Published in:The Journal of cell biology 2004-10, Vol.167 (1), p.161-170
Main Authors: Chen, Chun, Young, Bradford A., Coleman, Catherine S., Pegg, Anthony E., Sheppard, Dean
Format: Article
Language:English
Subjects:
Antibodies
Cell adhesion
Cell lines
CHO cells
Endothelial cells
Epithelial cells
Integrins
Polyamines
Small interfering RNA
Transfection
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Summary:The integrin α9β1 is expressed on migrating cells, such as leukocytes, and binds to multiple ligands that are present at sites of tissue injury and inflammation. α9β1, like the structurally related integrin α4β1, mediates accelerated cell migration, an effect that depends on the α9 cytoplasmic domain. α4β1 enhances migration through reversible binding to the adapter protein, paxillin, but α9β1-dependent migration is paxillin independent. Using yeast two-hybrid screening, we identified the polyamine catabolizing enzyme spermidine/spermine N1-acetyltransferase (SSAT) as a specific binding partner of the α9 cytoplasmic domain. Overexpression of SSAT increased α9β1-mediated migration, and small interfering RNA knockdown of SSAT inhibited this migration without affecting cell adhesion or migration that was mediated by other integrin cytoplasmic domains. The enzyme activity of SSAT is critical for this effect, because a catalytically inactive version did not enhance migration. We conclude that SSAT directly binds to the α9 cytoplasmic domain and mediates α9-dependent enhancement of cell migration, presumably by localized effects on acetylation of polyamines or of unidentified substrates.
ISSN:0021-9525