Occurrence of the 32-kDa Q B -binding protein of photosystem II in vegetative cells, heterocysts and akinetes of Azolla caroliniana cyanobionts

• Published in: Planta 1990-01, Vol.180 (3), p.361-371
• Main Authors: Braun-Howland, Ellen B., Nierzwicki-Bauer, Sandra A.
• Format: Article
• Language: English
• Subjects: Antibodies; Antiserum; Chloroplasts; Cyanobacteria; Glycogen; Hair cells; Plant cells; Plants; Somatic cells; Symbionts
• ISSN: 0032-0935; 1432-2048

Transmission electron microscopy and immunocytological labeling were used to localize the 32-kilodalton (kDa) protein (DI polypeptide) of photosystem II in different cell types of the cyanobionts within leaf cavities of Azolla caroliniana Willd. The 32-kDa protein binds the secondary electron acceptor QB, and is highly conserved between plants and cyanobacteria. Three antisera, specific for different epitopes of the 32-kDa protein, were used as primary antibodies. Immunologically recognizable 32-kDa protein was localized on membranes of Azolla chloroplasts, vegetative cyanobacterial cells, akinetes, and heterocysts that were at all stages of the differentiation process. The 32-kDa protein was not detected in nonphotosynthetic endosymbiotic bacteria found within leaf cavities. The amount of the 32-kDa protein observed in different cyanobacterial cell types was dependent upon the primary antiserum used and membrane orientation within a cell with respect to the plane of sectioning. Therefore, although 32-kDa protein was present in all three cyanobacterial cell types and clear trends in labeling patterns could be elucidated, it was not possible to quantitate the amounts of protein with respect to either cell type or leaf-cavity age.