Distinguishing Glyceria species of western North America

There are seven North American and three introduced European Glyceria species growing in western North America, yet distinguishing among the species is morphologically challenging. As contaminants in agronomic grass seed lots, the introduced species G. declinata and G. fluitans are undesirable domes...

Full description

Saved in:
Bibliographic Details
Published in:Seed technology 2009, Vol.31 (1), p.66-75
Main Authors: Bushman, B. Shaun, Sedegui, Mohammed, Osterbauer, Nancy K
Format: Article
Language:English
Subjects:
Biological taxonomies
Chloroplast DNA
Chloroplasts
DNA fingerprinting
DNA probes
gene amplification
Genetic hybridization
genetic markers
genetic variation
Glyceria
Glyceria borealis
Glyceria canademsis
Glyceria declinata
Glyceria elata
Glyceria fluitans
Glyceria grandis
Glyceria leptostachya
Glyceria maxima
Glyceria occidentalis
Glyceria striata
Herbaria
internal transcribed spacers
Introduced species
invasive species
molecular sequence data
phylogeny
plant taxonomy
Polymerase chain reaction
seed mixtures
Sequencing
Specimens
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:There are seven North American and three introduced European Glyceria species growing in western North America, yet distinguishing among the species is morphologically challenging. As contaminants in agronomic grass seed lots, the introduced species G. declinata and G. fluitans are undesirable domestically, while North American species are undesirable in seed grown for international trade. In order to distinguish between the western North American and introduced European Glyceria species we designed PCR, Taqman® SNP, and DNA sequencing assays. The PCR assays are co-dominant markers in which a larger sized amplification product is detected in G. declinata, G. fluitans, and G. fluitans-like G. occidentalis than is detected among the other species. The Taqman® SNP assay shows VIC hybridization signal for G. declinata and FAM hybridization signal for G. fluitans and G. fluitans-like G. occidentalis. DNA sequencing of the chloroplast trnK region and the nuclear ribosomal ITS-1 region provide several SNPs that identify each of the individual species. However, Glyceria occidentalis samples contain chloroplast and ITS-1 sequences identical to either G. leptostachya or G. fluitans, thus currently cannot be distinguished with DNA markers.
ISSN:1096-0724