Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing

• Published in: Proceedings of the National Academy of Sciences - PNAS 1992-02, Vol.89 (4), p.1301-1304
• Main Authors: Mayeda, A. (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY), Zahler, A.M, Krainer, A.R, Roth, M.B
• Format: Article
• Language: English
• Subjects: Alternative splicing; Amino Acid Sequence; Amino acids; Animals; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; Biochemistry; Biological and medical sciences; Complementary DNA; DROSOPHILA; Drosophila melanogaster; Family members; FOSFOPROTEINAS; Fundamental and applied biological sciences. Psychology; genes; Humans; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Multigene Family; Nuclear Proteins - isolation & purification; Nuclear Proteins - physiology; PHOSPHOPROTEINE; Phosphoproteins; Phosphoproteins - isolation & purification; Phosphoproteins - physiology; Phosphorylation; Proteins; Ribonucleic acid; RNA; RNA Splicing; RNA-Binding Proteins; Sequence Alignment; Serine-Arginine Splicing Factors; Splicing; SRp55 protein; Transcription. Transcription factor. Splicing. Rna processing; TRANSFERASAS; TRANSFERASE
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.89.4.1301

Monoclonal antibody 104 recognizes a subset of amphibian nuclear granules (B-snurposomes) and active sites of RNA polymerase II transcription in vertebrates and invertebrates. Monoclonal antibody 104 reacts with a set of nuclear serine- and arginine-rich phosphoproteins (SR family) with strikingly conserved apparent molecular masses. The most abundant family members in human (SRp33) and Drosophila (SRp55) cell lines can replace one another as essential splicing factors in a human cell-free system. Each of these polypeptides can functionally replace human SF2, an essential splicing factor that also regulates 5' splice site selection of alternatively spliced pre-mRNAs in vitro. Drosophila SRp55 also functions as an alternative splicing factor in the human cell-free system. Analysis of cloned cDNAs shows that SRp55 and SF2 are highly related and reveals regions of similarity to genetically defined regulators of alternative splicing in Drosophila. These results suggest that the conserved SR family of phosphoproteins, which includes SRp55 and SF2, is involved in constitutive pre-mRNA splicing and in the specificity of alternative splice site selection.